大豆原生质膜及混合细胞器膜脂脂肪酸对干旱胁迫的反应

本文分析了大豆复叶、真叶、子叶和发育子叶原生质膜及混合细胞器膜脂脂肪酸的组成、配比以及脂肪酸不饱和指数对干旱胁迫的反应。研究结果表明:大豆原生质膜及混合细胞器膜脂中主要有六种脂肪酸:豆蔻酸、棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸,还有少量的月桂酸和花生酸和一种未标记的脂肪酸。干旱胁迫不改变大豆不同叶类组织原生质膜和混合细胞器膜脂脂肪酸的组成,但配比和组分含量却发生了很大变化。脂肪酸组分中变化最显著的是亚麻酸,其次是亚油酸和棕榈酸。亚麻酸与脂肪酸不饱和指数呈极显著的正相关。耐旱型品种(庆选101)膜脂对干旱的反应,为脂肪酸的饱和化作用较慢,不饱和脂肪酸含量高于不耐旱品种(黑农11)。同一品种不同叶类对干旱的抗性表现的顺序为:复叶>真叶>发育子叶>子叶。

EFFECT OF DROUGHT ON FATTY ACID OF MEMBRANE LIPID OF PLASMIC MEMBRANCE AND MIX ORGANELLE IN SOYBEAN

In this paper we report the effect of drought on membranous fatty acid pattern, composition of membrane lipid and the index of unsaturated fatty acid (IUFA) in plasmic membrane and mix organelle from cotyledon, ture leaf, copmound leaf and cotyledon of developing seed of soybean. The results showed that the fatty acid composition of membrane lipid consisted of the myristic acid (14:0), stearic acid (16:0), palmitic acid (18:0), oleic acid (18:1),linolic acid (18: 2), linolenic acid (18:3), and a little lauric acid (12:0) and the arachidic acid (20:0) , and other kind of fatty acid. The fatty acid composition did not change during drought, but the content ratio of different fatty acids changed markedly. The change of the linolenic acid content was most obvions. The linolenic acid and the palmitic acid the second. There was significant positive correlation between the linolenic acid and the IUFA. The saturation of the fatty acid from the drought--resistant cultivar (Qing Xuan 101) was lower under drought condition, therefore, its content of the unsaturated fatty acid was higher than that of the drought--sensitive cultivar (Hei Hong 11). The sequence of drought-resistance of differeent leaf tissues of the same cultivar is: compound leaf > ture leaf > cotyledon of developing seed > cotyledon of seedling.