Discovery of host defence genes in the Japanese scallop Mizuhopecten yessoensis Jay by expressed sequence tag analysis of kidney tissue |
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| Authors: | Weidong Liu Chongbo He Wenji Li Zunchun Zhou Xianggang Gao Liyuan Fu |
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| Institution: | 1. Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China;2. Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China;3. College of Life Science, Liaoning Normal University, Dalian, China |
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| Abstract: | The Japanese scallop Mizuhopecten yessoensis is one of the most important aquaculture mollusca in Japan and China. In the present study, a high‐quality cDNA library of the Japanese scallop was constructed from the kidney tissue. A total of 2919 expressed sequence tags longer than 100 bp were generated from this library. A cluster of 1440 unique sequences, which consisted of 258 contigs and 1182 singletons, was revealed. Based on blast searches, 882 (61.3%) genes had significant (E‐value <1e–5) matches to known sequences in public databases. Among them, >70 genes were involved in stress response, immunity and apoptosis. These results expanded our knowledge of the genetics and physiology of the Japanese scallop, and provided a useful resource for gene discovery for further research of this species. |
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| Keywords: | Mizuhopecten yessoensis cDNA library expressed sequence tags immunity stress apoptosis |
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