Influence of the length of time after hormonal stimulation on selected parameters of milt of ide Leuciscus idus L. |
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| Authors: | Beata Irena Cejko Rados?aw Kajetan Kowalski Dariusz Kucharczyk Katarzyna Targońska S?awomir Krejszeff Daniel ?arski Jan Glogowski |
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| Institution: | 1. Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland;2. Department of Lake and River Fisheries, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury, Olsztyn, Poland;3. Department of Ichthyology, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury, Olsztyn, Poland |
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| Abstract: | Milt of the Leuciscus idus L. was collected from five experimental groups, and selected parameters of its quality were analysed for 36 h (group II), 60 h (group III), 84 h (group IV) and 108 h (group V), respectively, after hormonal stimulation with Ovopel (1 granule kg?1 of body weight). The control (group I) fish were not subjected to hormonal stimulation. The highest milt volume was obtained from the fish in group IV (0.70 ± 0.55 mL), where the largest volume of milt expressed per kilogram was also obtained (3.03 ± 1.94 mL kg?1). Significant differences were also found in milt volumes obtained between group I and groups III (P<0.01) and IV (P<0.05). The highest percentage of motile spermatozoa was found in the milt of group IV (59%); significant differences were found between group I and groups II (P<0.01) and III (P<0.001). The value of osmotic pressure of seminal plasma was the highest in group IV (203.19 ± 37.63 mOsm kg?1), and the lowest in group I (118.31 ± 41.13 mOsm kg?1). Parameters determining milt quality and quantity indicate that the period of 60–84 h after hormonal stimulation with Ovopel is optimal for obtaining milt from ide. |
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| Keywords: | Leuciscus idus L milt hormonal stimulation seminal plasma motility of spermatozoa Ovopel |
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