| 利用条形码技术鉴定苹浅褐卷蛾 |
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| 引用本文： | 和淑琪,查涛,蒋小龙,桂富荣,陈斌,李正跃.利用条形码技术鉴定苹浅褐卷蛾[J].植物检疫,2011(2). |
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| 作者姓名： | 和淑琪 查涛 蒋小龙 桂富荣 陈斌 李正跃 |
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| 作者单位： | 云南农业大学植物保护学院;云南出入境检验检疫局; |
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| 基金项目： | 国家自然科学基金项目(30860069) |
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| 摘 要： | 苹浅褐卷蛾原产澳大利亚,目前仅分布于少数地区,是澳大利亚、新西兰及英国等国家多种经济作物上的重要害虫,苹浅褐卷蛾形态具有多态性,对其形态鉴定一直非常困难,由此也增加了该虫在口岸上的入侵风险。本研究使用DNA条形码技术(barcoding)测定了采集自新西兰5个不同地区苹浅褐卷蛾种群26个样本的细胞色素酶I(COI)序列。研究表明,苹浅褐卷蛾种内COI序列差异远小于3%,而与BOLD数据库中卷蛾科其他种类的种间差异约为7%～16%,远大于3%。该结果肯定了条形码技术能够成为口岸快速、准确鉴定苹浅褐卷蛾的有效方法。
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| 关 键 词： | 卷蛾 外来入侵物种 生物安全 |
Identification of Epiphyas postvittana for biosecurity using DNA barcoding. |
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| He Shuqi,Zha Tao,Jiang Xiaolong,Gui Furong,Chen Bin,Li Zhengyue.Identification of Epiphyas postvittana for biosecurity using DNA barcoding.[J].Plant Quarantine,2011(2). |
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| Authors: | He Shuqi Zha Tao Jiang Xiaolong Gui Furong Chen Bin Li Zhengyue |
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| Institution: | He Shuqi1,Zha Tao2,Jiang Xiaolong2,Gui Furong1,Chen Bin1,Li Zhengyue1*(1.College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China,2.Yunnan Entry-Exit Inspection and Quarantine Bureau) |
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| Abstract: | Light brown apple moth(LBAM),Epiphyas postvittana,is a leafroller pest native to Australia and currently has limited distribution around the world.As a serious pest in its current distribution areas,it is well-known for its polymorphism with respect to its morphology.Morphological identification of E.postvittana has been problematic which increased the risk of it esacaping detection at the border of countries that wish to regulate this pest.In this study,we sequenced the COI gene from 26 samples of E.postvi... |
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| Keywords: | Tortricidae invasive species bio-security |
| 本文献已被 CNKI 等数据库收录！ |
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