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| 儿茶素低聚合物的MCI GEL分离及结构鉴定 | |
| 引用本文： | 尧渝,江和源,张建勇,刘洪云.儿茶素低聚合物的MCI GEL分离及结构鉴定[J].安徽农业科学,2010,38(16):8719-8722. |
| 作者姓名： | 尧渝 江和源 张建勇 刘洪云 |
| 作者单位： | 1. 中国农业科学院茶叶研究所,国家茶产业工程技术研究中心,农业部茶及饮料作物产品加工与质量控制重点实验室,浙江杭州,310008;中国农业科学院研究生院,北京,100089 2. 中国农业科学院茶叶研究所,国家茶产业工程技术研究中心,农业部茶及饮料作物产品加工与质量控制重点实验室,浙江杭州,310008 3. 中国农业科学院茶叶研究所,国家茶产业工程技术研究中心,农业部茶及饮料作物产品加工与质量控制重点实验室,浙江杭州,310008;烟台大学化学生物理工学院食品研究所,山东烟台,264005 |
| 基金项目： | 国家自然科学基金，农业部留学基金，教育部留学回国基金 |
| 摘 要： | 目的]分离儿茶素低聚合物,并对其结构进行鉴定。方法]利用MCI层析柱和甲醇水溶液洗脱体系,对茶多酚的氧化产物进行分离,并对分离后的各组分进行HPLC检测。结果]分离得到2种纯度相对较高的儿茶素低聚合物,经HPLC制备及结构鉴定,确定其中1种物质是黄酮苷类化合物。结论]该洗脱体系不仅可将氧化产物和简单儿茶素分离,也可使极性相近的氧化产物基本分离。 |
| 关 键 词： | 儿茶素 低聚合物 分离 MCIGEL 结构 |
Separation of Tea Catechin Oligomeric Products with MCI GEL and Its Structure Identification |
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| YAO Yu et al.Separation of Tea Catechin Oligomeric Products with MCI GEL and Its Structure Identification[J].Journal of Anhui Agricultural Sciences,2010,38(16):8719-8722. | |
| Authors: | YAO Yu |
| Institution: | YAO Yu et al(Key Laboratory of Processing and Quality Control of Tea and Beverage Plants,Ministry of Agriculture,National Engineering Technology Research Center for Tea Industry,Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou,Zhejiang 310008) |
| Abstract: | Objective] The research aimed to separate tea catechin oligomeric products with MCI GEL and identify its structure.Method] Tea oxidation products were fractionated by MCI column with 10% step-wise elution of H2O containing increasing proportions of MeOH.Result] HPLC spectrums showed that two catechin oligomeric products were separated with high purity,and one was identified to Kaempferol-3-O-β-D-glucoside.Conclusion] The study this fractionation not only can separate joxide from catechins,but also can separate oxidation products from each other. |
| Keywords: | MCI GEL |
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