| H5、H9亚型禽流感分子鉴别诊断方法的建立 |
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| 引用本文： | 黄淑坚,龚中贵,陈秀玲,梁小英,冼琼珍,马春全,王林川,辛朝安. H5、H9亚型禽流感分子鉴别诊断方法的建立[J]. 黑龙江畜牧兽医, 2007, 0(2): 22-24 |
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| 作者姓名： | 黄淑坚 龚中贵 陈秀玲 梁小英 冼琼珍 马春全 王林川 辛朝安 |
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| 作者单位： | 佛山科学技术学院生命科学学院 广东南海528231(黄淑坚，陈秀玲，梁小英，冼琼珍，马春全)，华南农业大学兽医学院 广东广州510642(龚中贵，王林川，辛朝安) |
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| 基金项目： | 广东省自然科学基金 , 广东省科技攻关计划 , 广东省佛山市专项基金 |
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| 摘 要： | 为了快速、敏感、特异地鉴别诊断禽流感,根据H5、H9亚型禽流感病毒HA基因序列的保守区域设计出并合成2对特异性引物,利用这2对引物对H5、H9亚型禽流感病毒的HA基因片段进行二联RT-PCR扩增,并对二联RT-PCR检测方法进行敏感性及特异性试验。结果表明:这2对引物能特异性地扩增出H5、H9亚型禽流感病毒HA基因片段,大小分别为490bp和686bp;该检测方法敏感性高,特异性强;初步建立了快速、敏感、特异地鉴别检测H5、H9亚型禽流感病毒的分子诊断方法。
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| 关 键 词： | 禽流感病毒 H5亚型 H9亚型 多联RT-PCR 检测 |
| 文章编号： | 1004-7034（2007）02-0022-03 |
| 收稿时间： | 2006-07-05 |
| 修稿时间： | 2006-07-05 |
Development of molecular method for differentiate detecting H5 and H9 subtype of Avian Influenza Virus |
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| HUANG Shu-jian ,GONG Zhong gui ,CHEN Xiu-ling ,et al. Development of molecular method for differentiate detecting H5 and H9 subtype of Avian Influenza Virus[J]. Heilongjiang Animal Science And veterinary Medicine, 2007, 0(2): 22-24 |
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| Authors: | HUANG Shu-jian GONG Zhong gui CHEN Xiu-ling et al |
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| Affiliation: | 1. College of Life Science, Foshan University, Nanhai 528231, China; 2. College of Veterinary Medicine, South China Agricultoral University, Guangzhou 510642, China |
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| Abstract: | Two pairs of specific primers were designed and synthesized according to conserved regions of HA genes of H5 and H9 subtype of avian influenza virus registered in GenBank.The results showed that,two specific bands,about 490 bp and 686 bp individually,were amplified by multiplex RT-PCR of H5 and H9 subtype of avian influenza virus with the two sets of specific primers. By specificity and sensitivity test,the detection method was highly specific and sensitive. The molecular differentiation method was preliminarily established to detect H5 and H9 subtype of avian influenza virus rapidly,sensitively and specifically. |
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| Keywords: | avian influenza virus H5 subtype H9 subtype multiplex RT-PCR detection |
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