| 响应面法优化灵芝胞外多糖的发酵工艺条件1） |
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| 引用本文： | 程俊文,;贺亮,;李肖娟,;胡传久,;魏海龙,;付立忠,;李海波. 响应面法优化灵芝胞外多糖的发酵工艺条件1）[J]. 中国林副特产, 2014, 0(3): 1-4 |
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| 作者姓名： | 程俊文, 贺亮, 李肖娟, 胡传久, 魏海龙, 付立忠, 李海波 |
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| 作者单位： | [1]浙江省林业科学研究院,浙江省森林资源生物与化学利用重点实验室,杭州310023; [2]杭州雪域生物技术有限公司,浙江省森林资源生物与化学利用重点实验室,杭州310023; |
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| 基金项目： | 杭州市农业科研攻关专项（20120232848）,浙江省科技厅院所专项（2012F30037）,浙江省食用茵育种专项（2012C12911-8）的部分研究内容 |
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| 摘 要： | 在单因素试验的基础上，通过Box-Benhnken组合设计和响应曲面分析法优化灵芝液体发酵产胞外多糖的发酵工艺条件。响应面分析结果表明，灵芝胞外多糖的最佳液体发酵培养条件为：葡萄糖15．79g/L ，玉米粉10．23g/L ，豆饼粉10．41g/L。此条件下的验证试验表明，此时胞外多糖的得率为340．05mg/L ，与理论值基本吻合。该方法对灵芝液体发酵多糖的开发与应用有重要意义。
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| 关 键 词： | 灵芝 发酵 胞外多糖 响应面 Response surface methodology (RSM ) |
Optimization of Culture Conditions for Production of Extracellular Polysaccharides by Ganoderma lucidum |
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| Affiliation: | Cheng Junwen, He Liang, Li Xiaojuan, Hu Chuanjiu, Wei Hailong, Fu Lizhong, Li Haibo (1. Zhejiang Forestry Academy, Key Laboratory of Biological and Chemical Utilization of Forest Resources , Hangzhon 310023;2. H angzhon Xneyu Biology technology corporation) |
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| Abstract: | Based on the results of one-factor-at-a-time-technique method ,a three-level Box-Behnken facto-rial design was employed combining with response surface methodology （RSM ） to optimize the conditions for extracellular polysaccharides （ EPS ） production by Ganoderma lucidum .The optimal fermentation conditions were determined as follows ：glucose 15 .79g/L , corn meal 10 .23g/L and soybean meal 10 .41g/L .The average yield of EPS validation experiments was 340 .05mg/L .This research has the vital significance regarding the Ganoderma lucidum polysaccharide function of the product development and the application1 . |
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| Keywords: | Box-Behnken Ganoderma lucidum Fermentation Extracellular polysaccharide Box-Behnken |
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