冷诱导基因CBF-4转化辣椒与转基因植株抗寒性分析 |
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引用本文: | 王兴娥,巩振辉,李大伟,陈儒钢,逯明辉,黄炜. 冷诱导基因CBF-4转化辣椒与转基因植株抗寒性分析[J]. 农业生物技术学报, 2009, 17(5): 830-835 |
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作者姓名: | 王兴娥 巩振辉 李大伟 陈儒钢 逯明辉 黄炜 |
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作者单位: | 西北农林科技大学园艺学院,杨凌,712100 |
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基金项目: | 国家自然科学基金项目,"十一五"国家科技支撑计划,"13115"科技创新工程重大科技专项,两北农林科技大学"青年学术骨干支持计划"资助 |
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摘 要: | 以4个辣椒(Capsicum annuum L. )品种为试材,利用根癌农杆菌介导的带柄子叶转化法将拟南芥CBF-4基因转入辣椒,通过调整预培养、共培养以及延迟培养时间等因素,优化并完善了甜椒品种B12的遗传转化体系,并对获得的60株卡那霉素抗性再生植株进行PCR和RT-PCR检测表明,46株为阳性转基因植株,转化率高达76.67%。经过低温处理后,对阳性植株的抗寒性生理、形态指标分析发现,转基因植株的SOD、POD和MDA活性都明显高于未转基因植株,转基因植株比未转基因植株的株型紧凑,叶片肥厚、平直,叶色浓绿,叶面积大。
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关 键 词: | 辣椒 遗传转化 农杆菌介导 CBF-4基因 |
收稿时间: | 2008-11-14 |
修稿时间: | 2009-01-23 |
Transformation of Pepper with Cold-induced Gene C- repeat Binding Factor 4 (CBF4) and Analysis of Cold Hardness-related Indexes |
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Abstract: | Four cultivars of pepper(Capsicum annuum L.)were successfully transformed with the CBF-4 gene by Agrobacterium mediated transformation. This study established a highly efficient regeneration system of pepper . The main factors influencing regeneration and transformation rate were discussed and the best conduction of regeneration and transformation were determined. The positive regeneraion was obtained.60 Km-resistant regenerated plants were obtained, 46 of which were positive by the analysis of PCR and RT-PCR.The rate of transgene is 76.67%.The morphological indexes of transgenetic plants showed that it was compact plant type with thicker, darker green and larger area leaves compared with the non-transgenetic plants. The enzyme activities of SOD, POD and MDA in transgenetic plants are higher than that of the non-transgenetic plant (control group) after the cold resistant treatment. |
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