冷诱导基因CBF-4转化辣椒与转基因植株抗寒性分析

以4个辣椒（Capsicum annuum L. ）品种为试材，利用根癌农杆菌介导的带柄子叶转化法将拟南芥CBF-4基因转入辣椒，通过调整预培养、共培养以及延迟培养时间等因素，优化并完善了甜椒品种B12的遗传转化体系，并对获得的60株卡那霉素抗性再生植株进行PCR和RT-PCR检测表明，46株为阳性转基因植株，转化率高达76.67%。经过低温处理后，对阳性植株的抗寒性生理、形态指标分析发现，转基因植株的SOD、POD和MDA活性都明显高于未转基因植株，转基因植株比未转基因植株的株型紧凑，叶片肥厚、平直，叶色浓绿，叶面积大。

Transformation of Pepper with Cold-induced Gene C- repeat Binding Factor 4 (CBF4) and Analysis of Cold Hardness-related Indexes

Four cultivars of pepper（Capsicum annuum L.）were successfully transformed with the CBF-4 gene by Agrobacterium mediated transformation. This study established a highly efficient regeneration system of pepper . The main factors influencing regeneration and transformation rate were discussed and the best conduction of regeneration and transformation were determined. The positive regeneraion was obtained.60 Km-resistant regenerated plants were obtained, 46 of which were positive by the analysis of PCR and RT-PCR.The rate of transgene is 76.67%.The morphological indexes of transgenetic plants showed that it was compact plant type with thicker, darker green and larger area leaves compared with the non-transgenetic plants. The enzyme activities of SOD, POD and MDA in transgenetic plants are higher than that of the non-transgenetic plant (control group) after the cold resistant treatment.