苹果潜隐病毒快速检测

该文以优系短枝富士为主要试材，采用木本指示植物和间接酶联免疫（ＰＡＳ－ＥＬＩＳＡ）两种方法，对苹果褪绿叶斑病毒（ＣＬＳＶ）、苹果茎沟病毒（ＳＧＶ）和苹果茎痘病毒（ＳＰＶ）进行了检测．试验表明，俄罗斯苹果Ｒ１２７４０－７Ａ、司派２２７和弗吉尼亚小苹果３种指示植物可作为上述３种潜在病毒的鉴定植物．植物生长状况对鉴定结果有重大影响．间接酶联免疫检测速度快，准确度高．该试验所用最佳抗体工作浓度ＣＬＳＶ：第一抗体１／６０００，第二抗体１／２０００；ＳＧＶ：第一抗体１／５０００，第二抗体１／３０００．

A Study on the Fast Detection of Latent Apple Viruses

Using excellent short twig Malus pumila cv.Fuji as main experimental material,the authors detected Apple Chlorotic Leafspot Virus,Apple Stem Grooving Virus and Apple Stem Pitting Virus by introducing indicator plant and indirect enzymelinked immunosorbent assay.It showed that Russia 12740 7A,SPY227 and Viginia crab can be used for the detection of the three latent viruses.The growth of indicator plant has great influence on the result.PAS ELISA has fast speed and high accuracy.The optimum working content of IgG:CLSV 1/6 000(first),1/2 000(second);SGV1/5 000(first),1/3 000(second).