| 棕榈科植物DNA的提取及SRAP引物筛选 |
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| 引用本文： | 林方养,杨耀东,万婕,唐嘉,马子龙,吴翼.棕榈科植物DNA的提取及SRAP引物筛选[J].江西农业学报,2014(7):115-117. |
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| 作者姓名： | 林方养 杨耀东 万婕 唐嘉 马子龙 吴翼 |
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| 作者单位： | [1]中国热带农业科学院椰子研究所/海南省热带油料作物生物学重点实验室,海南文昌571339 [2]琼州学院,海南五指山572200 [3]中国热带农业科学院热带生物技术研究所,海南海口571101 |
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| 基金项目： | 海南省自然科学基金（312039）. |
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| 摘 要： | 采用CTAB法提取棕榈科植物基因组DNA,选用4个棕榈科植物DNA作模板,对100个SRAP引物组合进行筛选,以条带清晰多态性丰富为引物筛选原则,最后共筛选出14个组合作为棕榈科植物SRAP分子标记的核心引物,为后续SRAP分析提供依据。同时还对SRAP-PCR扩增程序进行优化,在不影响扩增效果的前提下,节约了大量实验时间。
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| 关 键 词： | 棕榈科植物 DNA提取 引物筛选 |
Extraction of Genomic DNA from Palm Plants and Screening of SRAP Primers |
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| LIN Fang-yang,YANG Yao-dong,WAN Jie,TANG Jia,MA Zi-long,WU Yi.Extraction of Genomic DNA from Palm Plants and Screening of SRAP Primers[J].Acta Agriculturae Jiangxi,2014(7):115-117. |
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| Authors: | LIN Fang-yang YANG Yao-dong WAN Jie TANG Jia MA Zi-long WU Yi |
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| Institution: | 1. Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences ! Hainan Provincial Key Laboratory of Tropical Oil Crop Biology, Wenchang 571339, China; 2. Qiongzhou University, Wuzhishan 572200, China; 3. Institute of Tropi- cal Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China) |
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| Abstract: | The genomic DNAs were extracted from palm plants by using CTAB method , and 100 pairs of SRAP primers were screened for clear bands and rich polymorphism by using the extracted genomic DNAs from four palm plants as templates .Finally, 14 pairs of SRAP primers were screened out and were used as the core primers of palm plants SRAP molecular markers , which pro-vided the basis for the subsequent SRAP analysis in palm plants .This study also optimized the amplification program of SRAP -PCR, which could save much experimental time on the premise of not affecting the amplification effect . |
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| Keywords: | Palm plants DNA extraction Primer screening |
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