| 超声波法提取谷精草中总黄酮的条件优化 |
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| 引用本文： | 冯丽娟,;陈芳,;孙智勇,;张青峰,;王文君.超声波法提取谷精草中总黄酮的条件优化[J].江西农业学报,2014(6):84-86. |
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| 作者姓名： | 冯丽娟 ;陈芳 ;孙智勇 ;张青峰 ;王文君 |
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| 作者单位： | [1]遵义医学院珠海校区生物工程系,广东珠海519041; [2]江汉大学化学与环境工程学院,湖北武汉430056 |
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| 摘 要： | 以提取的总黄酮含量为考察指标,采用UV分光光度法测定其含量,通过正交试验考察乙醇浓度,超声时间及超声温度对提取率的影响,以得到最佳的提取条件。结果表明:各因素的影响程度为乙醇浓度>超声时间>超声温度,其中乙醇浓度和超声时间对提取率的影响最为显著,超声温度的影响不显著;最佳提取条件为:乙醇浓度为60%,超声温度为60℃,超声时间25 min;平均提取率为25.0 mg/g,RSD为0.4%。
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| 关 键 词： | 谷精草 总黄酮 超声波法 正交试验 |
Optimization of Conditions for Extraction of Total Flavonoids from Eriocaulon buergerianum by Ultrasonic Method |
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| Institution: | FENG Li-juan, CHEN Fang, SUN Zhi-yong, ZHANG Qing-feng, WANG Wen-jun FENG Li -juan , CHEN Fang, SUN Zhi -yong , ZHANG Qing -feng , WANG Wen -jun (1. Department of Bioengineering, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, China; 2. Department of Chemistry and Environmental Engineering, Jianghan University, Wuhan 430056, China) |
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| Abstract: | In order to gain the optimum conditions for the extraction of total flavonoids from Eriocaulon buergerianum Koern by ultrasonic method , we determined the content of extracted total flavonoids by using UV spectrophotometry , and investigated the influ-ences of ethanol concentration , ultrasonic temperature and ultrasonic time on the extraction yield of total flavonoids through orthogo -nal experiment .The results showed that the effect of various factors on the extraction yield revealed the following sequence ：ethanol concentration ＆gt;ultrasonic time ＆gt;ultrasonic temperature .Among these factors , ethanol concentration had the most significant in-fluence on the extraction yield , followed by ultrasonic time , while ultrasonic temperature had no significant influence .The best ex-traction conditions were gained as follows：60%ethanol , ultrasonic temperature 60℃, and ultrasonic time 25 min.Under the opti-mum conditions, the average extraction yield of total flavonoids was 25.0 mg/g, and the RSD was 0.4%. |
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| Keywords: | Eriocaulon buergerianum Total flavonoids Ultrasonic method Orthogonal experiment |
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