用超高效液相色谱-串联质谱法测定水体中32种药物

建立了采用超高效液相色谱-串联质谱法同时测定水中32种药物的方法,即:水样经稀硫酸酸化至pH 2.0,上样体积为500 mL,经HLB固相萃取小柱富集净化,超高效液相色谱-串联质谱仪测定,ESI正负离子同时扫描,以多反应监测(MRM)方式采集数据,外标法定量。各药物的平均加标回收率为61.4%~116.0%,RSD为0.7%~17.3%,检出限为0.4~2.9 ng/L。

Simultaneous Determination of 32 Drugs in Water Body by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

An ultra-high performance liquid chromatography -electrospray ionization tandem mass spectrometry （ UPLC-ESI-MS/MS） method was developed for the simultaneous determination of 32 drugs in water body .Firstly, the water sample was a-cidized to pH 2.0 by dilute sulphuric acid, and its volume was 500 mL;then the sample was richened and purified through solid -phase extraction with an Oasis HLB column;finally it was detected by using UPLC -ESI-MS/MS method.The ESI-MS/MS de-tection was achieved through the simultaneous scanning of ESI positive and negative ions , multiple reaction monitoring （ MRM） for data collection , and external standard method for quantitative analysis .The average standard -adding recovery of various drugs was 61.4%~116.0%, the RSD varied from 0.7%to 17.3%, and their detection limit was 0.4~2.9 ng/L.