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不同液体发酵条件对猪苓多糖提取量的影响
引用本文:戚淑威,赵琪,程远辉,侯志江,和琼姬,徐中志.不同液体发酵条件对猪苓多糖提取量的影响[J].江西农业学报,2014(2):115-117.
作者姓名:戚淑威  赵琪  程远辉  侯志江  和琼姬  徐中志
作者单位:云南省农业科学院高山经济植物研究所,云南丽江674100
摘    要:通过单因素试验,研究了不同液体发酵条件对猪苓多糖提取量的影响。研究结果表明:接种量、培养温度、振荡器转速、培养天数、培养基碳源、氮源及金属盐类等因素对猪苓多糖的提取量均有影响;当接种量为3片菌丝、培养温度为25℃、转速为150 r/min、培养15 d、培养基碳源为葡萄糖、氮源为蛋白胨、金属盐类为KH2PO4时,猪苓菌丝生长最好,猪苓多糖的提取量最高,达0.093~0.121 g。

关 键 词:猪苓  液体发酵  多糖  提取  条件

Effects of Different Conditions on Extraction of Polysaccharide from Grifola by Liquid Fermentation
QI Shu-wei,ZHAO Qi,CHENG Yuan-hui,HOU Zhi-jiang,HE Qiong-ji,XU Zhong-zhi.Effects of Different Conditions on Extraction of Polysaccharide from Grifola by Liquid Fermentation[J].Acta Agriculturae Jiangxi,2014(2):115-117.
Authors:QI Shu-wei  ZHAO Qi  CHENG Yuan-hui  HOU Zhi-jiang  HE Qiong-ji  XU Zhong-zhi
Institution:QI Shu-wei, ZHAO Qi, CHENG Yuan-hui, HOU Zhi-jiang, HE Qiong-ji, XU Zhong-zhi
Abstract:This article studied the effects of different conditions on the extraction of polysaccharide from grifola by liquid fer -mentation through single -factor experiments .The results showed that inoculum size , culture temperature , rotate speed of oscillator , culture duration , as well as carbon source , nitrogen source and metal salt in medium all had effects on the extraction of polysaccha -ride from grifola.When 3 pieces of mycelia were inoculated and cultured for 15 days at 25℃, the rotate speed was 150 r/min, and the medium contained glucose , peptone and KH 2 PO4 , the mycelia of grifola grew best , and the extraction quantity of grifola polysac-charide was the highest , reaching 0.093-0.121 g.
Keywords:Grifola  Liquid fermentation  Polysaccharide  Extraction  Condition
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