| 生发泡移植过程对小鼠卵母细胞体外受精的影响 |
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| 引用本文： | 崔龙波,周雪莹,崔志峰,黄秀英,孙方臻. 生发泡移植过程对小鼠卵母细胞体外受精的影响[J]. 河北农业大学学报, 2009, 32(4) |
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| 作者姓名： | 崔龙波 周雪莹 崔志峰 黄秀英 孙方臻 |
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| 作者单位： | 烟台大学,生物化学系,山东,烟台,264005;烟台大学,海洋学院,山东,烟台,264005;中国科学院,遗传与发育生物学研究所,北京,100080 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，山东省自然科学基金 |
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| 摘 要： | 为研究生发泡(GV)移植后的重组卵母细胞能充分在体外成熟和发育,本试验比较了GV移植过程及体外成熟对小鼠卵母细胞体外受精的影响。通过透明带切口及GV移植获得的重组GV期卵母细胞的成熟率为87.0%(147/169),体外受精后发育到原核期胚和2-细胞期胚的比率分别为76.2%(112/147)和42.2%(62/147),与仅做透明带切口但不进行GV移植的对照组相似(P>0.05),表明GV移植过程未对卵母细胞体外受精后的早期胚胎发育能力产生不利影响。但与体内成熟的卵母细胞相比,体外成熟卵母细胞的受精率和卵裂率都明显降低,表明其进一步发育的能力受到影响。
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| 关 键 词： | 卵母细胞 GV移植 体外成熟 体外受精 |
Effect of germinal vesicle translation process on in vitro fertilization of mouse oocytes |
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| CUI Long-bo,ZHOU Xue-ying,CUI Zhi-feng,HUANG Xiu-ying,SUN Fang-zhen. Effect of germinal vesicle translation process on in vitro fertilization of mouse oocytes[J]. Journal of Agricultural University of Hebei, 2009, 32(4) |
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| Authors: | CUI Long-bo ZHOU Xue-ying CUI Zhi-feng HUANG Xiu-ying SUN Fang-zhen |
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| Affiliation: | CUI Long-bo1,ZHOU Xue-ying1,CUI Zhi-feng2,HUANG Xiu-ying3,SUN Fang-zhen3 (1.Department of Biology,Yantai University,Yantai 264005,China,2.Ocean School,3.Institute of Genetics , Developmental Biology,Chinese Academy of Sciences,Beijing 100080,China) |
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| Abstract: | To study oocytes reconstructed by germinal vesicle(GV) translation can mature and develop fully in vitro,effects of GV translation process and in vitro maturation on in vitro fertilization(IVM) of mouse oocytes were compared.The maturation rate of the reconstructed oocytes obtained by zona cutting and GV translation was 87.0%(147/169),and the rates of pronuclear and 2-cell embryo developed from these oocytes after IVF were 76.2%(112/147)and 42.2%(62/147),respectively,which were comparable (P>0.05) with the ... |
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| Keywords: | oocyte GV translation in vitro maturation in vitro fertilization |
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