| 弹性硬蛋白溶解试验鉴别反刍动物腐蹄病节瘤拟杆菌强毒株 |
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| 引用本文： | 王克坚,陈立志.弹性硬蛋白溶解试验鉴别反刍动物腐蹄病节瘤拟杆菌强毒株[J].经济动物学报,1999,3(2):54-57. |
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| 作者姓名： | 王克坚 陈立志 |
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| 作者单位： | 中国农科院特产研究所，吉林农垦高等专科学校 |
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| 摘 要： | 根据腐蹄病节瘤拟杆菌毒力菌株具有溶解弹性硬蛋白的特性，利用弹性硬蛋白培养基建立了弹性硬蛋白溶解试验，以鉴别节瘤拟杆菌强毒株。试验表明，将不同毒力的菌株同时接种于弹性硬蛋白培养基，蛋白溶解带出现的时间和宽度都显著不同，强毒株大多在７ｄ内，少部分在７～１１ｄ内均出现一条明显的蛋白溶解带；而弱毒株在２１ｄ内基本上未见蛋白溶解带出现。
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| 关 键 词： | 腐蹄病 节瘤拟杆菌 弹性硬蛋白 |
Identification of Virulent Strains of D.nodosus by Elastase Activity in Agar Medium |
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| WANG Kejian,CHEN Lizhi,LIU Xiaoyinget al..Identification of Virulent Strains of D.nodosus by Elastase Activity in Agar Medium[J].Journal of Economic Animal,1999,3(2):54-57. |
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| Authors: | WANG Kejian CHEN Lizhi LIU Xiaoying |
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| Abstract: | The experimental results showed that cultures of virulent isolates invariably produced elastase and solubilized elastin in medium after 7 or 711 days'incubation,whereas cultures of benign isolates were elastase negative until 21 days'incubation,which means there was apparent correlation between elastase activity and virulence. |
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| Keywords: | Footrot D nodosus Elastase activity |
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