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| Bt晶体蛋白Cry1Ac放射免疫检测技术研究 | |
| 引用本文： | 潘家荣,乔艳红,张维,林敏,张杰.Bt晶体蛋白Cry1Ac放射免疫检测技术研究[J].核农学报,2006,20(6):544-547. |
| 作者姓名： | 潘家荣 乔艳红 张维 林敏 张杰 |
| 作者单位： | 1. 农业部农业核技术与农产品加工重点实验室,中国农业科学院农产品加工研究所,北京,100094 2. 中国农业科学院植物保护研究所,北京,100094 3. 中国农业科学院生物技术研究所,北京,100094 |
| 摘 要： | 通过苏云金芽孢杆菌(Bt)HD-73培养,提取Bt晶体蛋白Cry1Ac,经酶解后获得高抗虫活性蛋白。免疫新西兰白兔得到高纯度的Bt晶体蛋白1Ac抗体,用125I标记抗原,研究和建立了Bt晶体蛋白Cry1Ac的放射免疫检测技术,该试剂盒用磁性微粒作分离剂,不需离心即可分离,简化了测定步骤,检测结果达到国外同类产品(酶联免疫试剂盒)的水平。 |
| 关 键 词： | Bt Cry1Ac蛋白 放射免疫 转基因检测 |
| 文章编号： | 1000-8551(2006)06-544-04 |
| 收稿时间： | 08 25 2006 12:00AM |
| 修稿时间： | 2006年8月25日 |
STUDY ON RADIOIMMUNOASSAY OF Bt Cry1Ac PROTEIN |
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| PAN Jia-rong,QIAO Yan-hong,ZHANG Wei,LIN Min,ZHANG Jie.STUDY ON RADIOIMMUNOASSAY OF Bt Cry1Ac PROTEIN[J].Acta Agriculturae Nucleatae Sinica,2006,20(6):544-547. | |
| Authors: | PAN Jia-rong QIAO Yan-hong ZHANG Wei LIN Min ZHANG Jie |
| Abstract: | Bt Cry1Ac protein was extracted from incubation of Bacillus thuringiensis HD-73,and cutting into more specific protein segment with high insect-resistance.High-affinity multi-colonial antibodies of Bt Cry1Ac protein were obtained after injected it into New Zealand rabbits.By ~(125)I labeling of Bt Cry1Ac protein,a RIA kit was established.In this method,centrifuge for separation was not necessary due to the use of magnetic micro-particle and the specifications of the kit were found equal to those of imported ELISA. |
| Keywords: | Bt Cry1Ac protein radioimmunoassay detection of GMOs |
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