培养液组胺的检测及鲣鱼产组胺菌的生物活性评价 |
| |
引用本文: | 周秀锦,杨建,周向阳,沈飚,吴祖芳.培养液组胺的检测及鲣鱼产组胺菌的生物活性评价[J].浙江水产学院学报,2012(1):44-48. |
| |
作者姓名: | 周秀锦 杨建 周向阳 沈飚 吴祖芳 |
| |
作者单位: | [1]舟山出入境检验检疫局,浙江舟山316100 [2]应用海洋生物技术教育部重点实验室,宁波大学生命科学与生物工程学院,浙江宁波315211 |
| |
基金项目: | 浙江省公益技术研究社会发展项目(2010C33180) |
| |
摘 要: | 通过直接将培养液中的组胺经丹酰氯衍生化生成稳定的物质,以1,7-二氨基庚烷为内标,建立高效液相色谱-紫外检测器(HPLC-VWD)方法检测培养液中的组胺;并研究了温度和pH对产组胺菌生长和组胺产生的影响。结果表明,培养液中组胺的检测方法定量限为0.5μg/mL,回收率在100%~107%,满足方法培养液中组胺的检测。经分离得到的2株产组胺菌分别记为J2和J4,2株菌在添加L-组氨酸的胰酶大豆肉汤里均能够产生组胺;菌株J2和J4最适生长温度均为30℃,最适pH分别为7和6,菌株J2和J4产组胺的最适温度分别为25℃和30℃,最适pH均为5,在适宜的实验条件下组胺产生量最大值为12.47 mg/L。
|
关 键 词: | 培养液 组胺 检测 产组胺菌 生物活性 |
Detection Histamine in Nutrient Solution and Evaluation of Biological Characteristics of Histamine-Forming Bacteria in Raw Skipjack Tuna |
| |
Institution: | ZHOU Xiu-jin, YANG Jian, ZHOU Xiang-yang, et al (1. Entry-Exit Inspection and Quarantine Bureau, Zhoushan 316100; 2. Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China) |
| |
Abstract: | Histamine in nutrient solution is derived with dansyl chloride, determined by HPLC-VWD and quantified by isotope internal standard method with 1,7-Diaminoheptane. Histamine-forming bacteria were selected from the prescreening step using Actis's identification medium from skipjack tuna tissue, and true his-tamine formers were confirmed by HPLC method. Effects of temperature and pH on growth and histamine pro-duction of histamine-forming bacteria were studied. The results showed that the limit of determination of the method for nutrient solution is 0.5μg/mL, the recovery range is 100%-107%; two histamine formers isolated from skipjack tuna tissue were named as J2 and J4, which could produced histamine in trypticase soy broth supplemented with 1.0% L-histidine. The optimal temperature of growth of them were both 30 ℃, and the op- timal growth pH were 7 and 6, respectively. The optimal temperature of histamine production of them were 25 ℃ and 30 ℃, respectively. The optimal pH were both 5. The maximum of histamine reached 12.47 mg/L under the suitable culture condition. |
| |
Keywords: | nutrient solution detection histamine histamine-forming bacteria physiochemical properties |
本文献已被 维普 等数据库收录! |
|