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Role of Calcium Ion in Apoptosis of MD Cancer Cells Induced by Arsenic Trioxide |
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| Authors: | ZHANG Jiuli WANG Jintao XU Shiwen |
| Affiliation: | 1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;Heilongjiang Vocational College of Animal Husbandry and Veterinary Science, Shuangcheng 150100, Heilongjiang, China 2. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;Institute of Animal Science and Veterinary Medicine, Heilongjiang Academy of Land-Reclamation Sciences, Harbin 150038, China 3. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China |
| Abstract: | In order to observe the role of calcium ion in apoptosis of MD cancer cells induced by arsenic trioxide, inhibition percentage was detected by MTT assay;morphology changes were examined by fluorescence microscope;apoptosis was examined by DNA Ladder;[Ca2+]i was investigated by spectrofluorimeter in vitro on MDCC-MSB1 cells. The results showed that As2O3 inhibited the proliferation of MDCC-MSB1 cells in concentration dependent manner (P＜0.05 or P＜0.01);typical apoptosis character was observed by fluorescence microscope;DNA Ladder was observed;the [Ca2+]i was elevated significantly after the treatment of As203 (P＜0.05 or P＜0.01) and showed a dose-dependent manner. It is concluded that the calcium may play an important role in apoptosis of MD cancer cells induced by arsenic trioxide. |
| Keywords: | As2O3 MDCC-MSB1 apoptosis calcium ion |
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