| 玉米茎节再生体系建立及AGPL基因遗传转化的研究 |
| |
| 引用本文： | 张曼,吴颖,苏胜忠,李世鹏,单晓辉,刘宏魁,原亚萍. 玉米茎节再生体系建立及AGPL基因遗传转化的研究[J]. 玉米科学, 2016, 24(3): 51-56 |
| |
| 作者姓名： | 张曼 吴颖 苏胜忠 李世鹏 单晓辉 刘宏魁 原亚萍 |
| |
| 作者单位： | 吉林大学植物科学学院, 长春 130062;吉林省白城市农业科学院, 吉林 白城 137000,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062 |
| |
| 基金项目： | 国家转基因生物新品种培育重大专项(2009ZX08003-024B)、吉林省科技厅项目(20130206011NY) |
| |
| 摘 要： | 以玉米优良自交系郑58的子粒为材料,切取无菌苗的第1个茎节,上下各保留0.5 cm,接种于诱导培养基(1/2MS+0.3 mg/L NAA)培养,直接获得再生苗。腺苷二磷酸葡萄糖焦磷酸化酶(AGPase)是玉米淀粉合成过程中的一个限速酶,AGPase酶大亚基(AGPL)是酶的调节中心,在玉米胞质中过表达能够提高玉米淀粉含量。利用农杆菌介导法将玉米AGPL基因导入郑58的茎节体系,转化后的茎节在含有3mg/L双丙氨膦的1/2 MS培养基上进行筛选培养,对筛选后的抗性苗进行PCR和PCR-Southern blot检测,获得了4株转基因植株。
|
| 关 键 词： | 玉米 茎节 AGPL基因 遗传转化 |
| 收稿时间： | 2015-10-07 |
Establishment of Regeneration System and Transformation of Maize AGPL Gene in Maize Node |
| |
| ZHANG Man,WU Ying,SU Sheng-zhong,LI Shi-peng,SHAN Xiao-hui,LIU Hong-kui and YUAN Ya-ping. Establishment of Regeneration System and Transformation of Maize AGPL Gene in Maize Node[J]. Journal of Maize Sciences, 2016, 24(3): 51-56 |
| |
| Authors: | ZHANG Man WU Ying SU Sheng-zhong LI Shi-peng SHAN Xiao-hui LIU Hong-kui YUAN Ya-ping |
| |
| Affiliation: | College of Plant Science, Jilin University, Changchun 130062;Baicheng Academy of Agricultural Sciences, Baicheng 137000, China,College of Plant Science, Jilin University, Changchun 130062,College of Plant Science, Jilin University, Changchun 130062,College of Plant Science, Jilin University, Changchun 130062,College of Plant Science, Jilin University, Changchun 130062,College of Plant Science, Jilin University, Changchun 130062 and College of Plant Science, Jilin University, Changchun 130062 |
| |
| Abstract: | The maize nodes were used as the explants to develop a regeneration system through organogenesis on the basal 1/2MS media added with 0.3 mg/L NAA, and the upper and lower length of the node was 0.5 cm. ADP-glueose PyroPhosPhorylase(AGPase) was the key factor in the process of starch synthase. The large subunit of AGPase gene(AGPL) was the active center, Over expression of which could increase the starch content. The ZmAGPL gene was transferred into maize nodes mediated by Agrobacterium tumefaciens, and then the nodes were selected on the medium containing the concentration of bialaphos for 3 mg/L. Four transgenic plants of ZmAGPL gene were gained which were confirmed by PCR and PCR-Southern. |
| |
| Keywords: | Maize Node AGPL gene Transformation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《玉米科学》浏览原始摘要信息 |
|
点击此处可从《玉米科学》下载全文 |
|
