| 预苯酸脱氢酶和对羟基苯丙酮酸还原酶与野葛异黄酮的生物合成 |
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| 引用本文： | 李贺勤,杨华,张娟娟,宛晓春,方从兵.预苯酸脱氢酶和对羟基苯丙酮酸还原酶与野葛异黄酮的生物合成[J].安徽农业大学学报,2009,36(1). |
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| 作者姓名： | 李贺勤 杨华 张娟娟 宛晓春 方从兵 |
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| 作者单位： | 安徽农业大学园艺学院,合肥,230036;安徽农业大学农业部茶及药用植物安全生产重点开放实验室,合肥,230036;安徽农业大学园艺学院,合肥,230036;安徽农业大学农业部茶及药用植物安全生产重点开放实验室,合肥,230036 |
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| 基金项目： | 国家自然科学基金，安徽省优秀青年科技基金，安徽农业大学资助引进与稳定人才科研启动项目 |
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| 摘 要： | 以野葛悬浮细胞系为研究体系,测定PPDH酶和HPPR酶活性,并对细胞生长周期中2种酶活性与野葛异黄酮生物合成的相关性进行分析.结果表明,在野葛培养细胞中检测到PPDH酶的活性,并能催化预苯酸生成对羟基苯丙酮酸,由野葛培养细胞中提取的HPPR粗酶液能催化对羟基苯丙酮酸形成对羟基苯丙乳酸,从而在野葛细胞培养体系中证实了从预苯酸经对羟基苯丙酮酸到对羟基苯丙乳酸的反应.在野葛细胞培养周期中,随着PPDH酶和HPPR酶活性的提高,细胞培养体系中异黄酮的积累逐步增加,但异黄酮的积累滞后于酶活的提高;PPDH酶和HPPR酶活性与野葛异黄酮生物合成间存在较高的相关性, 相关系数分别为0.8812和0.8046.试验结果为以预苯酸为前体化合物,经对羟基苯丙酮酸和对羟基苯丙乳酸到香豆酸的中间代谢过程的一条异黄酮生物合成新途径的证实提供了初步的酶学研究证据.
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| 关 键 词： | 异黄酮 生物合成 预苯酸脱氢酶 对羟基苯丙酮酸还原酶 野葛 |
Roles of prephenate dehydrogenase and p-hydroxyphenylpyruvate reductase involved in isoflavonoid biosynthesis in cell suspension cultures of Pueraria lobata |
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| LI He-qin,YANG Hu,ZHANG Juan-juan,WAN Xiao-chun,FANG Cong-bing.Roles of prephenate dehydrogenase and p-hydroxyphenylpyruvate reductase involved in isoflavonoid biosynthesis in cell suspension cultures of Pueraria lobata[J].Journal of Anhui Agricultural University,2009,36(1). |
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| Authors: | LI He-qin YANG Hu ZHANG Juan-juan WAN Xiao-chun FANG Cong-bing |
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| Institution: | 1.School of Horticulture;Anhui Agricultural University;Hefei 230036;2.Key Laboratory of Tea & Medicinal Plants and Product Safety;Ministry of Agriculture;Hefei 230036 |
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| Abstract: | In this paper,the determination of the activities of prephenate dehydrogenase(PPDH) and p-hydroxyphenylpyruvate reductase(HPPR),and the correlations between the assaies of PPDH and HPPR and isoflavonoid accumulation in kudzu cell suspension culture were investigated,respectively.The results showed that PPDH activity was checked and PPDH crude enzyme from kudzu cell culture can catalyze the reaction from prephenate to p-hydroxyphenylpyruvate,and also HPPR crude enzyme can catalyze the reaction from p-hydroxy... |
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