鹅细小病毒主要结构蛋白VP3基因的克隆与序列分析

将GPV H2分离株接种于13日龄非免疫鸭胚，收集接毒后3－7日死亡鸭胚的尿囊液。纯化病毒，通过PCR技术，从病毒基因组DNA中扩增出病毒衣壳蛋白VP3完整基因片段，经酶切鉴定后直接与pMD 18-T质粒载体连接，转化感受态大肠杆菌TG1。提取重组质粒经PCR鉴定和酶切鉴定后，对插入片段进行序列测定及分析。结果表明；鹅细小病毒H1分离株VP3基因全长1605bp,编码534个氨基酸，与国外已发表的鹅细小病毒B株核苷酸序列同源性为98．5％，氨基酸序列同源性为98．3％，表明这二个毒株亲缘关系相近。

Cloning and Sequencing of the VP3 Gene of Goose Parvovirus

By Polymeraes chain reaction(PCR),VP3 gene was amplified from the DNA of goose parvovirus(H1),The PCR amplified VP3 gene was cloned into pMD 18_T vecter.The recombinant plasmid was identified with restriction endonuclease analysis and PCR,and then sequenced.The result of sequence analysis showed that the VP3 gene was 1605bp and included a complete open reading frame encoding a protein of 534 amino acids,and H1 isolate shared 98.5%and 98.3% identity with B isolate at nucleotide and amino acids level respectively.