RGA-ILP,a new type of functional molecular markers in bread wheat |
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Authors: | World Shang Ronghua Zhou Jizeng Jia Lifeng Gao |
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Institution: | (1) Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081 Beijing, China; |
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Abstract: | Markers targeting intron length polymorphism of resistance gene analogues (RGA-ILP) are not only expected to be more polymorphic
than those designed from conserved exons, but also have potential resistance gene function. Based on known domains of resistance
genes, more than 900 wheat RGAs were mined from public databases. Two hundred and seventy-eight intron-containing RGA candidates
were predicted based on rice genomic DNA information, and 50 of them were selected for evaluation and mapping. A total of
150 RGA-ILP primer pairs, consisting of exon-primed intron-crossing primers to amplify the intronic regions of RGAs were designed.
One hundred and thirty-five pairs were successfully amplified with similar or larger than expected product lengths. Three
mapping populations (SOpop, NYpop and WSpop) were screened and 28 pairs of RGA-ILP primers gave reproducible polymorphic amplifications
between the mapping parents. Sixteen, 14 and five loci were integrated into SOpop, NYpop and WSpop maps, respectively. The
results demonstrated that this method provides an efficient approach for developing polymorphic markers. |
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