| 鹿茸尖端组织核心蛋白多糖基因全长cDNA的克隆及其表达分析 |
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| 引用本文： | 郝丽.鹿茸尖端组织核心蛋白多糖基因全长cDNA的克隆及其表达分析[J].畜牧兽医学报,2011,42(6). |
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| 作者姓名： | 郝丽 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 黑龙江省自然科学基金，中央高校基本科研业务费专项资金资助 |
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| 摘 要： | 为了进一步了解鹿源DCN基因的结构与功能,揭示该基因在鹿茸尖端不同组织层的表达规律,本研究从梅花鹿鹿茸尖端组织cDNA文库中首次克隆具有完整编码区的DCN基因全长cDNA序列,并结合生物信息学方法和实时荧光定量RT-PCR技术对该基因的氨基酸序列结构和表达特征进行分析.结果表明,梅花鹿DCN基因cDNA全长为1 831 bp,编码360个氨基酸;其编码蛋白具有N端信号肽,相对分子质量为39.9 ku,理论等电点为8.8,其一级结构中亮氨酸所占比例最高(12.5%);梅花鹿与绵羊DCN氨基酸序列的相似性最高(98%).实时荧光定量RT-PCR分析表明,DCN在间充质层的表达显著高于其他3层组织,提示DCN的抗纤维化活性可能是维持鹿茸间充质层快速生长的重要调节因素.
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| 关 键 词： | 鹿茸 核心蛋白多糖 cDNA文库 克隆 实时荧光定量RT-PCR |
Full Length cDNA Cloning and Expression Analysis of DCN Gene from Velvet Tip Tissue |
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| HAO Li.Full Length cDNA Cloning and Expression Analysis of DCN Gene from Velvet Tip Tissue[J].Acta Veterinaria et Zootechnica Sinica,2011,42(6). |
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| Authors: | HAO Li |
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| Institution: | HAO Li(Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | This study was carried out to obtain and analyze sequence of deer DCN gene,and to reveal the expression of DCN mRNA in different tissues of velvet tip.The full length DCN cDNA from velvet tip tissue full-length cDNA library of Sika deer was isolated,bioinformatics method and Real-time RT-PCR technique were used to analyze the amino acid sequence and expression.The results showed that the full-length cDNA of DCN was 1 831 bp encoding a peptide of 360 amino acid residues,its relative molecular weight was 39.9... |
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| Keywords: | velvet Decorin(DCN) cDNA library clone Real-time RT-PCR |
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