乌梅酸枣仁提取物对鸡人工感染传染性法氏囊病的疗效试验

本试验旨在验证乌梅酸枣仁提取物对鸡传染性法氏囊病的预防和治疗效果。以鸡传染性法氏囊LX毒株感染未接种法氏囊疫苗的雏鸡，建立动物模型；以自拟乌梅酸枣仁提取物分高、中、低3个剂量组口服，以黄芪多糖为对照，评价其预防和治疗鸡传染性法氏囊病的疗效。结果表明：①人工感染鸡传染性法氏囊病毒后，预防试验中测试药物中剂量组、黄芪多糖组的相对增重率分别为81.85%和48.24%；成活率分别为87.5%和100%。统计分析结果表明，测试药物中剂量组的相对增重率显著高于黄芪多糖组（P<0.05），两组成活率差异不显著（P>0.05）；中剂量组、黄芪多糖组的法氏囊指数分别为0.187和0.123，脾脏指数分别为0.361和0.286。中剂量组的免疫器官指数均明显高于黄芪多糖组，差异显著（P<0.05）。②治疗试验中，测试药物中剂量组、黄芪多糖组的相对增重率分别为29.55%和46.1%，成活率分别为100%和80%。中剂量组、黄 芪多糖组的法氏囊指数分别为0.22和0.25，脾脏指数分别为0.34和0.36。经过统计学分析，上述两试验组的各项指标差异均不显著（P>0.05）。由此可知，乌梅酸枣仁提取物以200 mg/kg体重口服，连续5 d能有效提高人工感染传染性法氏囊病鸡的成活率，且预防和治疗保护率均超过80%；测试药物通过提高鸡法氏囊、脾脏指数而发挥增强机体免疫功能抵抗病毒侵害的功效。

Curative Effects of Fructus Mume and Semen Ziziphi Spinosae Extracts against IBD

To assess the pharmacological effects of compound of Fructus mume and Semen ziziphi spinosae extracts against infectious bursal disease (IBD) in the laboratory. Firstly, the chickens without the attenuated IBD vaccine were inoculated with the IBDV LX strain in order to establish the virus disease model. Secondly,Fructus mume and Semen ziziphi spinosae extracts were formulated according to the previous study. Chickens were administered orally with 400,200 and 100 mg/kg herb extracts.The chickens were administered with astragalus polysaccharide as the control group.All birds were monitored daily and evaluated the efficacy.The results showed that: ①After experimental infection with IBD,average weight gain of the infected chickens was 81.85% in 200 mg/kg group and 48.24% in astragalus polysaccharide group. As for survival rate, the infected chickens were 87.5% and 100% in above group,respectively.Average weight gain was significant difference in 200 mg/kg group as compared to that of astragalus polysaccharide group. No significance was found between two groups. The chicken bursal index was 0.187 and 0.123, respectively in 200 mg/kg group and in astragalus polysaccharide group while the spleen index were 0.361 and 0.286, respectively in above two groups. There was significant difference (P<0.05) between two groups.②In second experiment, the relative weight gain in above mentioned groups were 29.55 and 46.1 while survival rate was 100% and 80%, respectively, which was no significant difference between 200 mg/kg group and astragalus polysaccharide group. The chicken bursal index were 0.22 and 0.25 while spleen index were 0.34 and 0.36, respectively. Statistical analysis showed that no significant difference was found in immune organ index between two mentioned groups (P>0.05). Chickens were orally administered with 200 mg/kg Fructus mume and Semen ziziphi spinosae extract for 5 days, resulting in maximum 80% survival rate against the artificially challenge with IBD. The improvement of immune organ index contributed to the promising pharmacological effects.