| 鹅细小病毒非结构蛋白B细胞线性抗原表位的鉴定及抗原表位区分子特性分析 |
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| 引用本文： | 于天飞,仇铮,马波,高明春,张文龙,王君伟.鹅细小病毒非结构蛋白B细胞线性抗原表位的鉴定及抗原表位区分子特性分析[J].中国预防兽医学报,2011,33(8). |
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| 作者姓名： | 于天飞 仇铮 马波 高明春 张文龙 王君伟 |
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| 作者单位： | 1. 东北农业大学动物医学学院,黑龙江哈尔滨150030;齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006
2. 齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔,161006 |
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| 基金项目： | 黑龙江十五攻关项目(GB01B503-02、GB04B504) |
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| 摘 要： | 为进一步对鹅细小病毒(GPV)非结构(NS)蛋白B细胞线性抗原表位进行定位,本研究设计了覆盖NS1蛋白C末端453 aa～627 aa的7个长约30个氨基酸残基的重叠短肽,并进行了融合表达。蛋白质印迹分析结果表明,表达的融合蛋白NSb(485 aa～514 aa)、NSc(498 aa～532 aa)、NSd(523 aa～556 aa)、NSe(543 aa～573 aa)、NSf(564 aa～598 aa)和NSg(599 aa～627 aa)能够被GPV免疫的鹅血清识别。将抗原表位区推导氨基酸序列与GenBank中登录的12株GPV和番鸭细小病毒(MDPV)相应序列应用DNAMAN进行同源性比较,并构建了系统进化树。分析结果表明,GPV各株之间的同源性较高,但在强弱毒株和不同地区分离株中存在一定的差异。同时,GPV和MDPV的NS蛋白中可能存在共同的线性抗原表位区。
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| 关 键 词： | 鹅细小病毒 非结构蛋白 抗原表位 分子特性 |
Identification of linear B-cell epitopes on goose parvovirus non-structural protein and the gene sequence analysis |
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| YU Tian-fei,QIU Zheng,MA Bo,GAO Ming-chun,ZHANG Wen-long,WANG Jun-wei.Identification of linear B-cell epitopes on goose parvovirus non-structural protein and the gene sequence analysis[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(8). |
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| Authors: | YU Tian-fei QIU Zheng MA Bo GAO Ming-chun ZHANG Wen-long WANG Jun-wei |
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| Institution: | YU Tian-fei1,2,QIU Zheng1,MA Bo1,GAO Ming-chun1,ZHANG Wen-long1,WANG Jun-wei1*(1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China,2.College of Life Science and Agricultural and Forestry,Qiqihar University,Qiqihar 161006,China) |
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| Abstract: | In order to determining the B-cell epitopes on the non-structural protein(NS) of goose parvovirus(GPV),Seven DNA fragments were amplified by RT-PCR,and expressed in E.coli,which encoded 7 partially overlaying short peptides covering the C-terminal of NS protein(453aa to 627aa).The western blot analysis results show that the NS protein linear B-cell epitopes were located on 485aa to 514aa,498aa to 532aa,523aa to 556aa,543 to 573aa,564aa to 598aa and 599aa to 627aa.The deduced amino acid of epitope sequence w... |
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| Keywords: | goose parvovirus non-structural protein epitope sequence analysis |
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