| 宠物源大肠杆菌的血清型和毒力基因及耐药性调查 |
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| 引用本文： | 张美君,廖晓萍,王秀梅,朱恒乾,孙坚,孙迎,王杨,庄娜,刘雅红.宠物源大肠杆菌的血清型和毒力基因及耐药性调查[J].中国预防兽医学报,2011,33(8). |
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| 作者姓名： | 张美君 廖晓萍 王秀梅 朱恒乾 孙坚 孙迎 王杨 庄娜 刘雅红 |
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| 作者单位： | 1. 华南农业大学兽医学院广东省兽药研制与安全评价重点实验室,广东广州,510642
2. 中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金-广东省政府自然科学基金联合基金重点项目(U0631006); 教育部创新团队项目(IRT0723) |
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| 摘 要： | 为研究宠物源致病性大肠杆菌(Escherichia coli)的血清型、毒力基因和耐药性之间的相关性,本研究由健康和患病犬、猫直肠拭子样品中分离177株E.coli,并采用玻片凝集法鉴定其血清型,结果显示分离株中定型菌株135株,分别属于20个不同的血清型,其中O1、O2和O8为主要的流行血清型。PCR方法检测11种毒力相关基因,并采用琼脂稀释法测定分离菌株对14种抗菌药的敏感性,结果表明3个血清型的菌株拥有相似的耐药表型,但毒力基因谱不同。62%的分离菌株携带fimH基因,并且毒力基因组合iroN+hlyF、iroN+fimH和traT+sitA比较流行。55%的O1血清型携带fimH基因,并且多数耐受四环素、多西环素、头孢噻吩和庆大霉素;20.8%的O2血清型菌株携带traT和sitA基因;50%的O8血清型携带traT和fimH基因。3个血清型分离菌株多数对安普霉素和阿米卡星敏感。
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| 关 键 词： | 大肠杆菌 宠物源 血清型 毒力基因 耐药性 |
Prevalence of serotypes,virulence genes and antimicrobial susceptibility of Escherichia coli isolated from pets |
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| ZHANG Mei-jun,LIAO Xiao-ping,WANG Xiu-mei,ZHU Heng-qian,SUN Jian,SUN Ying,WANG Yang,ZHUANG Na,LIU Ya-hong.Prevalence of serotypes,virulence genes and antimicrobial susceptibility of Escherichia coli isolated from pets[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(8). |
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| Authors: | ZHANG Mei-jun LIAO Xiao-ping WANG Xiu-mei ZHU Heng-qian SUN Jian SUN Ying WANG Yang ZHUANG Na LIU Ya-hong |
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| Institution: | ZHANG Mei-jun1,LIAO Xiao-ping1,WANG Xiu-mei2,ZHU Heng-qian1,SUN Jian1,SUN Ying1,WANG Yang1,ZHUANG Na1,LIU Ya-hong1*(1.College of Veterinary Medicine,Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation,South China Agricultural University,Guangzhou 510642,China,2.Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 100051,China) |
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| Abstract: | To investigate the E.coli isolated from pets in Guangzhou,the islates were primarily test by agglutination with specific O serogroup antisera.Twenty O serogroups were identified for 135 E.coli isolates,of which O1,O2 and O8 were dominant serogroups.Virulence genes detection by PCR method showed that 62% of the isolates possessed fimH gene and most of the E.coli isolates with a combinations of iroN+hlyF,iroN+fimH and traT+sitA.Furthmore,55% of O1 serogroup carried fimH gene and most of O1 isolates were resis... |
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| Keywords: | Escherichia coli pet resources serogroups virulence genes antimicrobial resistance |
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