| 水稻田中铜抗性微生物的定量研究 |
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| 作者姓名： | LI Xiao-Fang YIN Hong-Bin SU Jian-Qiang |
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| 作者单位： | Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361003 (China);Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, the University of Queensland, St Lucia Qld 4072 (Australia);Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China);Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361003 (China) |
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| 基金项目： | Supported by the International Copper Association, Rio Tinto Limited, the Nickel Producers Environmental Research Association, the Key International S&T Cooperation Projects of China (No. 2009DFB90120);the National Natural Science Foundation of China (No. 31070101) |
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| 摘 要： | Understanding the mechanisms of Cu pollution-induced community tolerance (PICT) in soil requires the characterization of Cu-resistant microorganisms at a community level using modern molecular tools. A primer pair (copAF2010 (5 -TGCAC CTGAC VGGSC AYAT-3 )/copAR2333 (5 -GVACT TCRCG GAACA TRCC-3 )) tentatively targeting Pseudomonas-like Cu-resistant microorganisms was designed in this study. The specification of the primers was tested through conventional polymerase chain reaction (PCR) and the construction of a Pseudomonas-like copA gene fragment library, and then the primers were used to quantify the Cu-resistant microorganisms using quantitative PCR technique. A significant increase of Cu-resistant microorganisms targeted by the primers was observed in a paddy soil from Jiaxing, China which has been exposed to one-year Cu contamination. The results provided direct evidence for Cu PICT in the soil, and the quantification method developed in this study has the potential to be used as a molecular assay for soil Cu pollution.
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| 关 键 词： | copA Cu contamination pollution-induced community tolerance (PICT) quantitative PCR |
| 收稿时间： | 27 May 2011 |
An attempt to quantify Cu-resistant microorganisms in a paddy soil from Jiaxing, China |
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| LI Xiao-Fang,YIN Hong-Bin,SU Jian-Qiang.An attempt to quantify Cu-resistant microorganisms in a paddy soil from Jiaxing, China[J].Pedosphere,2012,22(2):201-205. |
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| Authors: | LI Xiao-Fang YIN Hong-Bin and SU Jian-Qiang |
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| Institution: | Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361003 (China)
;Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, the University of Queensland, St Lucia Qld 4072 (Australia);Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China);Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361003 (China) |
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| Abstract: | Understanding the mechanisms of Cu pollution-induced community tolerance (PICT) in soil requires the characterization of Cu-resistant microorganisms at a community level using modern molecular tools. A primer pair (copAF2010 (5'-TGCAC CTGAC VGGSC AYAT-3')/copAR2333 (5'-GVACT TCRCG GAACA TRCC-3')) tentatively targeting Pseudomonas-like Cu-resistant microorganisms was designed in this study. The specification of the primers was tested through conventional polymerase chain reaction (PCR) and the construction of a Pseudomonas-like copA gene fragment library, and then the primers were used to quantify the Cu-resistant microorganisms using quantitative PCR technique. A significant increase of Cu-resistant microorganisms targeted by the primers was observed in a paddy soil from Jiaxing, China which has been exposed to one-year Cu contamination. The results provided direct evidence for Cu PICT in the soil, and the quantification method developed in this study has the potential to be used as a molecular assay for soil Cu pollution. |
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| Keywords: | copA Cu contamination pollution-induced community tolerance (PICT) quantitative PCR |
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