|
|||||
|
|
| 绵羊卵母细胞的孤雌激活 | |
| 引用本文： | 施巧婷,王旭光,王立虎,杨梅,郭志勤. 绵羊卵母细胞的孤雌激活[J]. 中国畜牧杂志, 2004, 40(6): 10-13 |
| 作者姓名： | 施巧婷 王旭光 王立虎 杨梅 郭志勤 |
| 作者单位： | 1. 新疆畜牧科学院绵羊中心,新疆乌鲁木齐,830000 2. 新疆农业大学动物科技学院,新疆乌鲁木齐,830052 3. 北京农学院生物技术系,北京,102206 |
| 摘 要： | 本文探讨了不同激活方法对绵羊卵母细胞的孤雌激活和其后的发育。结果表明 ,电激活可以激活绵羊卵母细胞孤雌发育到囊胚 ;Ca2 + 载体A2 3187和CHX组合 ,Ionomycin和 6 DMAP组合可以激活绵羊卵母细胞 ,其卵裂率与电激活相比差异显著。 7%乙醇激活绵羊卵母细胞 7min效果较好。不同场强、不同脉冲次数对绵羊卵母细胞激活都有影响 ,以 1.2kV/cm ,间隔 30 μs和 3次脉冲效果较好。而电激活与化学激活联合可以更好的激活绵羊卵母细胞 |
| 关 键 词： | 畜牧学 孤雌激活 卵母细胞 绵羊 |
| 文章编号： | 0258-7033(2004)06-0010-04 |
| 修稿时间： | 2003-05-15 |
Parthenogenetic Activation of Ovine Oocytes |
|
| SHI Qiao-ting,WANG Xu-guang,WANG Li-hu,YANG Mei,GUO Zhi-qin. Parthenogenetic Activation of Ovine Oocytes[J]. Chinese Journal of Animal Science, 2004, 40(6): 10-13 | |
| Authors: | SHI Qiao-ting WANG Xu-guang WANG Li-hu YANG Mei GUO Zhi-qin |
| Affiliation: | SHI Qiao-ting~1,WANG Xu-guang~2,WANG Li-hu~3,YANG Mei~1,GUO Zhi-qin~1 |
| Abstract: | In this article, we discussed the effect of different activation methods on parthenogenetic development of ovine oocytes. The results showed that calcium ionophore cycloheximide, ionomycin 6-dimethylaminopurine, electric pulse or alcohol could activate the ovine oocytes. And 7% alcohol could activate ovine IVM oocytes for 7 min. Ovine oocytes were treated by 1.2 kV/cm and 3 pulses electric activation had high activation rate. When the oocytes were primed with electric pulse then cultured in media containing A-23187-CHX or Io-6-DMAP, the activation rate were significantly higher than that stimulated with electric pulse or A-23187-CHX, Io-6-DMAP alone. |
| Keywords: | Animal science Parthenogenesis Oocyte Ovine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |