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一种微生态制剂中抑菌物质的分离与鉴定
引用本文:陈有容,李淑侠,等.一种微生态制剂中抑菌物质的分离与鉴定[J].上海水产大学学报,2001,10(4):333-337.
作者姓名:陈有容  李淑侠
作者单位:陈有容(上海水产大学食品学院,上海,200090)       李淑侠(上海水产大学食品学院,上海,200090)       齐凤兰(上海水产大学食品学院,上海,200090)
摘    要:植物乳杆菌发酵产生的抑菌物质经乙醇浸提、SephadexG-15层析、氨基酸组成分析、反相液相色谱分离及鉴定,发现该植物乳杆菌产生的抑菌物质为一类分子量较小(小于1000Da),且比较接近,极性不同的多肽。

关 键 词:抑菌物质  植物乳杆菌  凝胶色谱  反相色谱  微生态制剂  发酵
文章编号:1004-7271(2001)04-0333-05
修稿时间:2001年6月25日

Purification and identification of antibacterial substance in a kind of microecological modulator
CHEN You rong,LI Shu xia,QI Feng lan.Purification and identification of antibacterial substance in a kind of microecological modulator[J].Journal of Shanghai Fisheries University,2001,10(4):333-337.
Authors:CHEN You rong  LI Shu xia  QI Feng lan
Abstract:The Lactobacillus plantarum studied was previously found to produce wide spectrum antibacterial substance. Purification methods of the antibacterial substance were studied in this paper. Ethanol extraction, sephadex G 15 gel chromatography and reverse phase chromatography were used to purify the antibacterial substance. Amino acid composition of the antibacterial substance was detected. According to the result,it could be concluded that the L. Plantarum produced several kinds of low weight molecular, polypeptide like antibacterial substance.
Keywords:antibacterial substance  L  plantarum  gel chromatography  reverse phase chromatography
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