蒿属药用植物叶绿体上的psbA-trnH序列分析

【摘要】 目的：采用叶绿体上psbA-trnH序列中的信息，运用物种特异的DNA序列信息位点区分黄花蒿和常见同属植物的混淆种茵陈蒿、青蒿，为药用植物的鉴定提供科学依据。方法：提取被测植物的DNA，使用PCR方法扩增psbA-trnH序列片段，测定并比较所得序列，软件分析得到上述三种植物各自特有的DNA序列特征位点。结果：黄花蒿和茵陈蒿、青蒿植物之间psbA-trnH序列虽具有较高的相似性，但也有显著的特征性的不同，可以依靠它们各自特有的信息位点区分开来。结论：psbA-trnH序列中的信息位点可以用来区分黄花蒿、茵陈蒿和青蒿植物。该方法首次为蒿属药用植物的分子鉴定提供了可靠、简便的参考依据。

psbA-trnH sequence analysis from chloroplast on medicinal plants of Artemisia

Artemisia annua L.is a traditional Chinese herbal medicine used in the treatment of malaria disease.But the dry samples of Artemisia capillaris Thunb.and Artemisia carvifolia Buch.are also used as A.annua in local areas.The adulterants are similar to A.annua and it is difficult to identify the botanical origin of these herbs.This research focused on analyzing the differences of psbA-trnH region sequences of A.annua,A.capillaris and A.carvifolia.The result showed that although the psbA-trnH regions among the three species are high similar; there are several character-sites for themselves,which can be used in discriminating them.It is the first time to use DNA technology in drug species' discrimination of Artemisia,which provided a reliable and simple reference for the authentication of them.