| Damas 1869李组培繁殖技术研究 |
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| 引用本文： | 许建兰,马瑞娟,俞明亮,沈志军.Damas 1869李组培繁殖技术研究[J].果树学报,2008,25(5):740-743,F0003. |
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| 作者姓名： | 许建兰 马瑞娟 俞明亮 沈志军 |
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| 作者单位： | 江苏省农业科学院园艺研究所,南京210014 |
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| 基金项目： | 农业行业科研专项基金，科技部科技基础条件平台建设计划 |
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| 摘 要： | 以Damas1869李腋芽为材料,研究了不同消毒方法对冬季水培芽及春季田间芽污染率、存活率的影响。采用3因素2水平正交实验,研究不同激素配比对芽增殖的影响,不同浓度的IBA、NAA对生根的影响。结果表明,与春季田间芽相比以冬季水培芽灭菌效果较好,即以75%酒精浸洗10s,再用20%次氯酸钠浸洗8min,存活率为64.1%;最佳芽增殖培养基是MS+6-BA1.0mg/L+NAA0.5mg/l+20g/L蔗糖+5g/L琼脂粉,增殖系数最高可达7.1;适宜生根的培养基是1/2MS+IBA2.0mg/L+20g/L蔗糖+5g/L琼脂粉+0.05%活性炭,生根率为87.8%,平均生根数3.9。
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| 关 键 词： | Damas1869李 组织培养 |
Studies on the tissue culture of Damas 1869 plum |
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| XU Jian-lan,MA Rui-juan,YU Ming-liang,SHEN Zhi-jun.Studies on the tissue culture of Damas 1869 plum[J].Journal of Fruit Science,2008,25(5):740-743,F0003. |
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| Authors: | XU Jian-lan MA Rui-juan YU Ming-liang SHEN Zhi-jun |
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| Institution: | XU Jian-lan,MA Rui-juan,YU Ming-liang,SHEN Zhi-jun(Institute of Horticulture,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014 China) |
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| Abstract: | Experiment was carried out using the buds of winter shoots advanced by water culture method in room and the spring shoots directly from the field of Damas1869 as material for evaluating the effect of different sterilization on contamination and survival rate in vitro.The effects of different hormones on bud proliferation by 3 factors and 2 levels,the effect of IBA and NAA on rooting were studied.Results showed that the buds of winter shoot were better used as explants than those of spring shoots.The best me... |
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| Keywords: | Damas1869 plum Tissue culture |
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