家蚕表皮蛋白基因的生物信息学分析

家蚕的表皮蛋白(cuticular protein)是家蚕重要的结构蛋白,与几丁质一同作为家蚕表皮的重要组成部分。运用生物信息学的方法对家蚕表皮蛋白进行了广泛分析。通过保守的基序检索家蚕基因组,得到163条候选的表皮蛋白序列,包括RR、Tweedle、CPF&CPFL等家族,并且发现具有确定的保守基序的表皮蛋白基因在染色体上都是以串联集群形式分布。氨基酸组成分析表明,这些表皮蛋白富含甘氨酸、丙氨酸、亮氨酸、组氨酸等。运用Sig-nalP server预测这部分蛋白质有85%都具有信号肽。进化分析显示,负选择是家蚕表皮蛋白基因进化的主要驱动力。采用TFSEARCH等在线服务软件分析发现,在70%含有RR基序的家蚕表皮蛋白基因的核心启动子区具有通用的TATAAA序列。

Bioinformation Analysis of Cuticular Protein Genes in the Silkworm, Bombyx mori

Cuticular proteins are important structural proteins for the silkworm,Bombyx mori,and play essential roles in formation of cuticle.By searching the conserved motif in silkworm genome draft,we identified 163 genes encoding putative cuticular proteins,which harbors RR consensus,Tweedle motif,CPF & CPFL family features,and so on.These genes are distributed in the form of gene clusters on chromosomes.We further analyzed amino acid composition of 163 genes and found that Glycine,Alanine,Leucine,and Histidine are prominent residues.Eighty-five percent of the 163 cuticular proteins have a signal peptide by SignalP server prediction.In addition,the results of evolutionary analysis indicated that the diversification of cuticular protein genes was driven by strong negative selection.Moreover,70% of core promoter regions of RR cuticular protein genes share the common TATAAA sequence by searching the online server TFSEARCH.