| 黄波斯菊试管苗开花技术研究 |
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| 引用本文： | 黄志明,刘才文. 黄波斯菊试管苗开花技术研究[J]. 江西农业学报, 2011, 23(3) |
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| 作者姓名： | 黄志明 刘才文 |
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| 作者单位： | 莆田学院环境与生命科学系,福建莆田,351100 |
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| 基金项目： | 福建省莆田市科技计划资助项目 |
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| 摘 要： | 不同基本培养基对黄波斯菊试管苗成花试验结果表明:黄波斯菊的种子用0.1%升汞消毒8 min的效果最好,成活率为92%;最佳的增殖培养基配方为MS+6-BA0.2 mg/L+NAA0.02 mg/L+蔗糖20 g/L+琼脂10 g/L;芽苗在1/2 MS+NAA0.2 mg/L+PP3330.5 mg/L+糖20 g/L+琼脂10 g/L培养基中培养,植株表现为矮壮、叶片呈浓绿色、根系发达。取健壮的植株在改良MS+PP3330.5 mg/L+6-BA1.0 mg/L+糖20 g/L+琼脂10 g/L培养基上培养35～45 d后即可开花。
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| 关 键 词： | 黄波斯菊 试管苗 开花 培养基 |
Studies on Flowering Technology of Test-tube Plantlets of Cosmos sulfureus |
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| HUANG Zhi-ming,LIU Cai-wen. Studies on Flowering Technology of Test-tube Plantlets of Cosmos sulfureus[J]. Acta Agriculturae Jiangxi, 2011, 23(3) |
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| Authors: | HUANG Zhi-ming LIU Cai-wen |
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| Affiliation: | HUANG Zhi-ming; LIU Cai-wen(Department of Environment & Life Science; Putian University; Putian 351100; China); |
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| Abstract: | The flowering technology of test-tube plantlets of Cosmos sulfureus was studied in this paper.The results showed that disinfecting the seeds of Cosmos sulfureus with 0.1% HgCl2 for 8 minutes achieved the highest survival percentage(92%).The best proliferation medium was MS+ 6-BA 0.2 mg/L+ NAA 0.02 mg/L+ sucrose 20 g/L+ agar 10 g/L.The 1/2 MS basic medium supplemented with NAA 0.2 mg/L,PP333 0.5 mg/L,sugar 20 g/L and agar 10 g/L was found to be the best for the development of buds,producing short and strong ... |
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| Keywords: | Cosmos sulfureus Test-tube plantlet Flowering Medium |
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