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SDS-CTAB法提取烟草病圃土壤微生物总DNA
引用本文:蔡刘体,胡重怡,罗正友.SDS-CTAB法提取烟草病圃土壤微生物总DNA[J].江西农业学报,2011,23(2):119-121.
作者姓名:蔡刘体  胡重怡  罗正友
作者单位:贵州省烟草科学研究所,贵州,贵阳,550081
基金项目:贵州省烟草专卖局项目"烟草病圃微生物多样性分析及烟草抗性基因研究",贵州省科技厅项目"烟草青枯病病原菌天敌的筛选及生防体系研究"
摘    要:采用SDS-CTAB法提取了烟草病圃土壤中微生物的总DNA,经过纯化后获得的DNA大小约为21 kb,OD260/OD280高于1.70;用细菌和真菌保守序列引物进行PCR扩增,琼脂糖凝胶电泳能检测到预期大小的扩增条带。

关 键 词:烟草  病圃  土壤微生物  总DNA  SDS-CTAB法

Extraction of Total DNA of Microbes from Tobacco Diseased-field Soil by SDS-CTAB Method
CAI Liu-ti,HU Zhong-yi,LUO Zheng-you.Extraction of Total DNA of Microbes from Tobacco Diseased-field Soil by SDS-CTAB Method[J].Acta Agriculturae Jiangxi,2011,23(2):119-121.
Authors:CAI Liu-ti  HU Zhong-yi  LUO Zheng-you
Institution:(Guizhou Tobacco Science Research Institute,Guiyang 550081,China)
Abstract:In this paper,the total DNA was extracted from the soil microbes of tobacco diseased-fields by SDS-CTAB method.The size of the obtained DNA after purification was about 21 kb,and the value of OD260/OD280was higher than 1.70.The PCR amplification with bacterial and fungal conserved sequence primers showed that the amplified bands with the expected size could be detected by agarose gel electrophoresis.
Keywords:Tobacco  Diseased-field  Microbes in soil  Total DNA  SDS-CTAB method
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