| 鸭源大肠杆菌3种tet基因的多重PCR检测 |
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| 引用本文： | 许瑞,刘建华,胡功政,吴华,陈玉霞,赵金凤,孟春萍.鸭源大肠杆菌3种tet基因的多重PCR检测[J].江西农业学报,2011,23(3):150-152. |
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| 作者姓名： | 许瑞 刘建华 胡功政 吴华 陈玉霞 赵金凤 孟春萍 |
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| 作者单位： | 河南农业大学,牧医工程学院,河南,郑州,450002 |
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| 摘 要： | 利用肉汤稀释法测定21株鸭大肠杆菌对四环素等7种抗茵药物的敏感性,并用多重PCR方法同时检测3种四环素耐药基因(tetA、tetB和tetC).结果显示:所有菌株均呈现tetA、tetB和tetC基因阳性,与药敏试验结果阳性符合率76.67%,携带3种tet基因的临床分离鸭大肠杆茵不仅对四环素类药物耐药,还对头孢噻肟、...
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| 关 键 词： | 多重PCR 鸭大肠杆菌 四环素耐药基因 tet基因 |
Multiplex PCR for Detection of Three Tetracycline-resistant Genes in E.coli Isolated from Duck |
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| XU Rui,LIU Jian-hua,HU Gong-zheng,WU Hua,CHEN Yu-xia,ZHAO Jin-feng,MENG Chun-ping.Multiplex PCR for Detection of Three Tetracycline-resistant Genes in E.coli Isolated from Duck[J].Acta Agriculturae Jiangxi,2011,23(3):150-152. |
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| Authors: | XU Rui LIU Jian-hua HU Gong-zheng WU Hua CHEN Yu-xia ZHAO Jin-feng MENG Chun-ping |
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| Institution: | XU Rui; LIU Jian-hua; HU Gong-zheng; WU Hua; CHEN Yu-xia; ZHAO Jin-feng; MENG Chun-ping(College of Animal Science and Veterinary Medicine; Henan Agricultural University; Zhengzhou 450002; China); |
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| Abstract: | The MICs of 7 antibacterial agents against E.coli isolates(n=21) from duck were determined by using the broth microdilution method,and 3 tetracycline-resistant genes(tetA,tetB and tetC) were detected simultaneously by multiplex PCR.The results showed that all isolates showed positive results of three tet genes,the result of PCR amplification was 76.67% identical with that of the drug resistance test.The isolates containing three tet genes were resistant to tetracycline,cefotaxime,enrofloxacin and florfenico... |
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| Keywords: | Multiplex PCR E coli from duck Tetracycline-resistant gene |
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