非洲菊组织培养中La3+的应用初探

 以非洲菊‘Sunbird’品种的花托、幼叶、幼芽为外植体, 研究不同浓度La³⁺对愈伤组织形成、生长、幼芽分化增殖的影响。结果表明, 低浓度La³⁺对花托愈伤组织形成、生长及芽的分化有促进作用,最佳处理浓度为10 mg/L , 处理浓度大于10 mg/L 时抑制芽的分化, 并明显引起褐化。La³⁺对离体叶片愈伤组织形成生长的促进作用亦随处理浓度的提高而增大, 但愈伤组织的褐化率也增大, 最佳处理浓度为15 mg/L。幼芽的增殖系数在La³⁺ 0～50 mg/L 范围内随浓度的提高而增大, 50 mg/L 时最大, 达20. 8 , 但浓度大于30 mg/L 时增殖过程易出现玻璃化苗并引起叶畸形, 增殖的最佳浓度为30 mg/L。

Effects of La3 + on Tissue Culture of Gerbera jasmesonil ‘ Sunbird'

Effects of La 3+ on the callus formation and growth ,bud initiation and multiplication of Gerbera jasmesonil `sunbird' were studied. Floral receptacle, leaf and bud explants were inoculated on the media with different concentration of La 3+. The result showed that La 3+ could stimulate the callus formation and growth,and the bud differentiation at low concentration,the suitable concentration was 10mg/L in receptacle explants and over this concentration, the bud initiation was inhibited and much more explants turned browning.In leaf explants, the suitable concentration for callus formation and growth was 15mg/L,and 30mg/L for bud initiation and multiplication respectively.