| 马鹿粪便样品微卫星基因型的可靠性评价 |
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| 引用本文： | 田新民,张明海.马鹿粪便样品微卫星基因型的可靠性评价[J].安徽农业科学,2010,38(15):8279-8280. |
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| 作者姓名： | 田新民 张明海 |
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| 作者单位： | 牡丹江师范学院生物系,黑龙江牡丹江,157012;东北林业大学野生动物资源学院,黑龙江哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金项目，黑龙江省自然科学基金重点项目，美国老虎和犀牛基金资助项目，牡丹江师范学院教改规划项目 |
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| 摘 要： | 目的]评价马鹿粪便DNA的微卫星基因型可靠性,为后续保护遗传学方面的研究提供参考。方法]选择7个多态性较高的微卫星位点,扩增167份马鹿粪便DNA,通过3～4次的重复扩增统计等位基因丢失率和假等位基因出现率。结果]7个位点的等位基因丢失率平均为0.034,假等位基因出现率平均为0.023。结论]对于杂合体,3～4次的重复扩增可以满足基因型后续分析要求,而纯合体需要7次以上的重复扩增。
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| 关 键 词： | 马鹿 粪便样品 微卫星 基因型 |
Assessing Reliability of Microsatellite Genotypes from Wapiti Fecal Samples |
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| TIAN Xin-min et al.Assessing Reliability of Microsatellite Genotypes from Wapiti Fecal Samples[J].Journal of Anhui Agricultural Sciences,2010,38(15):8279-8280. |
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| Authors: | TIAN Xin-min |
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| Institution: | TIAN Xin-min et al(Department of Biology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012) |
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| Abstract: | Objective]The aim was to assess reliability of microsatellite genotypes from wapiti fecal DNA in order to offer researching reference for conservation genetics in the future.Method]Using 7 high variable loci,167 fecal DNA were genotyped,the ratio of allele dropout and false allele by 3-4 times repeat amplification was estimeated.Result]The average ratio for allele dropout was 0.034 in 7 loci,the false allele was 0.023.Conclusion]The method of 3-4 times repeat amplification may be used to assess the geno... |
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| Keywords: | Wapiti Fecal sample Microsatellite Genotype |
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