玉米抗甘蔗花叶病毒资源的遗传多样性研究

利用人工接毒方法对46份我国主要玉米自交系进行了两年抗甘蔗花叶病毒鉴定,筛选出高抗系8份(K22、CN962、P138、齐318、中自01、金黄96B、齐319、Pa405),抗病系7份(旱21、中自03、旱23、农大178、获白、K12、黄早四).用SSR标记研究了46份自交系的遗传多样性.49对引物共检测出168个等位基因变异,每对引物检测等位基因2～10个,

Genetic Diversity of Elite Maize Germplasm for Resistant to SCMV

Forty six elite maize( Zea mays L.) inbred lines were evaluated for reaction to sugarcane mosaic virus under field conditions with artificial inoculation in 1999 and 2000. The results showed that 8 lines (K22, CN962, P138, Qi318, Zhongzi01, Jinhuang96B, Qi319, and Pa405)were highly resistant to SCMV,and 7(Han21, Zhongzi03, Han23, Nongda178, Huobai, K12,and Huangzao4)were resistant. The germplasm basis for resistance to SCMV in 46 inbreds was investigated by SSR markers. The average number of alleles per SSR locus was 3.43 with a range from 2 to 10. The UPGMA cluster analysis showed that the 46 inbreds could be classified into 7 distinct groups with several subgroups, which were generally consistent to their known pedigree information and breeder's experiences. Fifteen resistant inbreds were found in 4 groups (A,B,E,G), which could be used in germplasm improvement for SCMV resistance based on heterotic pattern, respectively. Group E and subgroup GII were identified as two important sources of resistance, which can be employed to synthesize the composites for SCMV resistance. The study provided useful information for germplasm improvement for resistance to SCMV.