番茄基因组研究进展

【目的】综述国内外相关高质量番茄基因组研究进展,比较与分析第一、二、三代测序技术的研究成果,为番茄重要性状功能基因的挖掘及新品种选育提供参考。【方法】收集查阅国内外相关官网、文献资料和现有研究前沿技术,整理汇总并进行对比,分析番茄基因组测序技术研究进展。【结果】栽培番茄S. lycopersicum Heinz 1706基因组利用一代和二代测序技术8年完成测序和组装。3个野生番茄基因组采用二代测序技术3年完成,三代测序技术缩短了测序时间,并且在二代测序技术的基础上提高了基因组的质量,提供了完整性高达96.46%的野生番茄品种潘那利的参考基因组。【结论】测序技术推动了番茄基因组研究,三代测序技术与一、二代技术相比,在测序速度、基因序列读长和准确性方面显著提高,也使基因组组装更加精确。

Advances in Tomato Genome Research

【Objective】 As an important vegetable crop, tomato is a model plantform genetic research. The development of sequencing technology will lay a solid foundation for the excavation of important functional genes in tomato.【Method】 High quality of tomato genome information by analyzing and comparing the relative websites、researches and technologies will provide a reliable research strategy for breeders. 【Result】The development of sequencing technology has greatly promoted tomato genome research. It took eight years for sequencing and assembling the genome of cultivated tomato S. lycopersicum Heinz 1706 by first and second generation of sequencing technology. However, three wild tomato species was sequenced by second generation of sequencing technology only within three years. Comparing with the second generation of sequencing technology, the third generation of sequencing technology not only shorten the sequencing time, but also improved the genome quality by providing wild tomato S. pennellii genome sequence with higher integrity of 96.46%.【Conclusion】From the comparison of genome sequencing technology, we gained the conclusion that the third generation of sequencing technology has greatly improved the sequencing speed, the reading length and the accuracy of the genome sequencing. At the same time, it makes the genome assembly more accurate. The decoding of tomato genome information will open a new way for tomato researchers gained new varieties of tomato.