| 甜菜细胞质雄性不育系与其保持系线粒体DNA的RAPD分析 |
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| 引用本文： | 田自华,张子义,张剑峰,史树德,白薇,邵金旺. 甜菜细胞质雄性不育系与其保持系线粒体DNA的RAPD分析[J]. 分子植物育种, 2004, 2(6): 817-822 |
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| 作者姓名： | 田自华 张子义 张剑峰 史树德 白薇 邵金旺 |
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| 作者单位： | 内蒙古农业大学甜菜生理研究所,呼和浩特,010018;内蒙古甜菜制糖工业研究所,呼和浩特,010070;浙江大学生命科学学院,杭州,310029 |
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| 摘 要： | 本文对甜菜线粒体DNA的提取方法进行了改进，在不需密度梯度离心条件下即可获得高纯度的DNA。优化了甜菜线粒体的RAPD的反应条件，对细胞质雄性不育甜菜的不育系及其保持系的线粒体DNA(mtDNA)进行了RAPD(随机扩增多态DNA，randomly amplified polymorphic DNA)分析，结果表明：甜菜细胞质雄性不育系和保持系线粒体DNA之间存在丰富的多态性。
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| 关 键 词： | 甜菜 细胞质雄性不育 线粒体DNA RAPD |
RAPD Analysis of Mitochondrial DNA of Cytoplasmic Male Sterile Line and it's Maintainer Line in Sugarbeet |
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| Tian Zihua Zhang Ziyi Zhang Jianfeng Shi Shude Bai Wei Shao Jinwang Sugarbeet Physiological Institute,Inner Mongolia Agricultural University,Hohhot, Sugarbeet and Sugar Industry Institute of Inner Mongolia,Hohhot, College of Life Sciences,Zhejiang University,Hangzhou. RAPD Analysis of Mitochondrial DNA of Cytoplasmic Male Sterile Line and it's Maintainer Line in Sugarbeet[J]. Molecular Plant Breeding, 2004, 2(6): 817-822 |
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| Authors: | Tian Zihua Zhang Ziyi Zhang Jianfeng Shi Shude Bai Wei Shao Jinwang Sugarbeet Physiological Institute Inner Mongolia Agricultural University Hohhot Sugarbeet Sugar Industry Institute of Inner Mongolia Hohhot College of Life Sciences Zhejiang University Hangzhou |
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| Affiliation: | Tian Zihua1* Zhang Ziyi1 Zhang Jianfeng2 Shi Shude3 Bai Wei1 Shao Jinwang11 Sugarbeet Physiological Institute,Inner Mongolia Agricultural University,Hohhot,0100182 Sugarbeet and Sugar Industry Institute of Inner Mongolia,Hohhot,0100703 College of Life Sciences,Zhejiang University,Hangzhou,310029 |
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| Abstract: | Mitochondrial DNA (mtDNA) extracted from cytoplasmic male sterile(CMS) line and its maintainer line of sugarbeet was studied by means of random amplified polymorphic DNAs(RAPD). The fingerprints of the amplification by polymerase chain reaction (PCR) of random DNA segments was analysised. The results indicate that there are the obvirous difference between CMS line and maintainer line revealed by RAPD technique. Meanwhile this test improved the extraction method of mtDNA also optimized the program of RAPD. |
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| Keywords: | Sugarbeet CMS mtDNA RAPD |
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