| Abstract: | Objective] To establish a rapid,fast and accurate high performance liquid chromatographic analysis for HERBA TAXILLI.Methods]Quercitrin and quercetin were extracted from HERBA TAXILLI by acid hydrolysis and ultrasonic extraction. Quercitrin and quercetin contents were detected by RP-HPLC. Results] Quercetin was the main component extracted by acid hydrolysis. There were few free quercetins by ultrasonic extraction; and they were mainly existed in the form of quercitrin. Conclusions] Quercitrin extracted by ultrasonic extraction was used as the index for quantitative determination; detection was carried out by RP-HPLC. This method could rapidly and accurately reflect the quality of HERBA TAXILLI. |
