In Vitro Fertilization and Development of
Porcine Oocytes Matured in Follicular Fluid |
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| Authors: | Budiyanto AGUNG Takeshige OTOI Dai-ichiro FUCHIMOTO Shoichiro SENBON Akira ONISHI Takashi NAGAI |
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| Institution: | 1)NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan;2)The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8511, Japan;3)Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan |
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| Abstract: | This study was conducted to assess the fertilization and development of porcine oocytes
matured in a solo follicular fluid (pFF) using different in vitro culture
systems and insemination periods. Cumulus-oocyte complexes (COCs), follicular cells (FCs),
and pFF were collected from the follicles of ovaries. The pFF was used as a maturation
medium (MpFF) after supplementation with follicle stimulating hormone (FSH) and
antibiotics. The COCs were matured in a 15 ml test tube containing 3.5 ml of MpFF with FCs
(5.2 × 106 cells/ml; rotating culture system) or 2 ml of MpFF without FCs in a
35-mm petri dish (static culture system) for 44 to 48 h. After maturation culture, oocytes
were co-incubated with frozen-thawed spermatozoa for 5 h and then cultured for 7 days. The
total mean rates of sperm penetration, normal fertilization, male pronucleus (MPN)
formation, cleavage, and development to the blastocyst stage of oocytes after insemination
were significantly higher (P<0.01) in the rotating culture system than in the static
culture system. In conclusion, compared with the static culture system, the rotating
culture system is adequate for the production of developmentally competent porcine oocytes
when MpFF is used as a maturation medium. |
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| Keywords: | Embryonic development Follicular fluid In vitro maturation Pigs Rotating culture |
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