| Abstract: | Objective] To compare the fingerprint features of different varieties of FLOS CHRYSANTHEMI,and to lay foundation for the establishment of HPLC fingerprint chromatogram of FLOS CHRYSANTHEMI. Methods] HPLC fingerprint chromatograms of different varieties of FLOS CHRYSANTHEMI were analyzed,which were collected from Tongxiang Germplasm Resources Garden in Zhejiang Province. The correlations of different varieties of FLOS CHRYSANTHEMI were researched by similarity analysis,cluster analysis and principal component analysis. Results] HPLC fingerprint chromatograms of FLOS CHRYSANTHEMI were established,showing that there was certain similarity in HPLC chromatograms of different varieties. A total of 13 common peaks were screened. Results of fingerprint data,cluster analysis and principal component analysis showed the connection of different varieties of FLOS CHRYSANTHEMI in regionality and genetic relationship,as well as their unique characteristics. Conclusions]The chromatographic system could be used for establishing the fingerprints of different varieties of FLOS CHRYSANTHEMI,which provided references for the application,quality evaluation and germplasm selection of FLOS CHRYSANTHEMI. |
