Good Thermally Conducting Material Supports Follicle Morphologies of
Porcine Ovaries Cryopreserved with Ultrarapid Vitrification |
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Authors: | Shu HASHIMOTO Nao SUZUKI Ami AMO Takayuki YAMOCHI Yoshihiko HOSOI Yoshiharu MORIMOTO |
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Institution: | 1)IVF Namba Clinic, Osaka 5500015, Japan;2)School of Biology-Oriented Science and Technology, Kinki University, Wakayama 6496493, Japan;3)St. Marianna University School of Medicine, Kanagawa 2168512, Japan |
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Abstract: | Effects of supporting materials during vitrification procedure on the morphologies
of preantral follicles of pig ovaries were assessed. Ovarian cortical sections of
prepubertal pigs were randomly allocated to 5 groups. The sections were vitrified
ultrarapidly with 5 different vitrification devices. The sections were put on 4 fine
needles (Cryosupport), on a thin copper plate, or on a carbon graphite sheet or were
sandwiched between copper plates or between carbon graphite sheets before cooling.
The cooling and warming rates with the graphite sheets were significantly higher than
those with the copper plates (P<0.05). A total of 3,064 follicles were analyzed
following HE staining after vitrification with 5 different devices. The morphologies
follicles vitrified on the Cryosupport or on the graphite sheet were well preserved
compared with those vitrified on the copper plate or between copper plates
(P<0.01). The morphologies of follicles vitrified between copper plates were
mostly damaged (P<0.05). Taken together, good thermally conducting material
supports follicle morphologies of ovaries cryopreserved with ultrarapid
vitrification. |
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Keywords: | Cryopreservation Fertility preservation Ovary Ultrarapid vitrification |
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