Production of Diabetic Offspring Using Cryopreserved Epididymal Sperm by
In Vitro Fertilization and Intrafallopian
Insemination Techniques in Transgenic Pigs |
| |
| Authors: | Kazuhiro UMEYAMA Kasumi HONDA Hitomi MATSUNARI Kazuaki NAKANO Tatsuro HIDAKA Keito SEKIGUCHI Hironori MOCHIZUKI Yasuhiro TAKEUCHI Tsukasa FUJIWARA Masahito WATANABE Masaki NAGAYA Hiroshi NAGASHIMA |
| |
| Affiliation: | 1)Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan;2)Meiji University International Institute for Bio-Resource Research, Kanagawa 214-8571, Japan |
| |
| Abstract: | Somatic cell nuclear transfer (SCNT) is a useful technique for creating pig strainsthat model human diseases. However, production of numerous cloned disease model pigsby SCNT for large-scale experiments is impractical due to its complexity andinefficiency. In the present study, we aimed to establish an efficient procedure forproliferating the diabetes model pig carrying the mutant human hepatocyte nuclearfactor-1α gene. A founder diabetes transgenic cloned pig was generated by SCNT andtreated with insulin to allow for normal growth to maturity, at which pointepididymal sperm could be collected for cryopreservation. In vitrofertilization and intrafallopian insemination using the cryopreserved epididymalsperm resulted in diabetes model transgenic offspring. These results suggest thatartificial reproductive technology using cryopreserved epididymal sperm could be apractical option for proliferation of genetically modified disease model pigs. |
| |
| Keywords: | Cryopreserved epididymal sperm Diabetes model pigs Intrafallopian insemination In vitro fertilization Transgenic offspring |
|
