醉蝶花离体培养植株再生研究

 诱导醉蝶花下胚轴和子叶形成愈伤组织, 以MS + 6-BA 0.5 mg/L + NAA 0.5 mg/L + KT 0.5 mg/L培养基中子叶的出愈率最高, 达到100 % , 下胚轴出愈率最高只有93 %。MS + 6-BA 2 mg/L + NAA 0.05 mg/L最适合于芽的诱导, 诱导率达到100 %。再生苗在MS + NAA 0.1 mg/L 培养基中生根完成植株再生。带单个侧芽的茎作为外植体接种在MS + 6-BA 1 mg/L + NAA 0.05 mg/L 培养基中, 一个月以后形成无数丛生芽。

Studies on Plant Regeneration of Cleome spinosa Jacq.in Vitro

Taking MS as basic media,adopting 6-BA,NAA,KT of different concentration compoundings to induce callus from cotyledons and hypocotyls of Cleome spinosa Jacq.,the result showed that the medium of MS 6-BA 0.5 mg/L NAA 0.5 mg/L KT 0.5 mg/L was the best to induce callus from cotyledons with a rate of 100% frequency,and the highest rate of hypocotyl callus inducement was 93%.And the differentiation medium MS 6-BA 2 mg/L NAA 0.05 mg/L was the best to induce buds and had a 100% plant regeneration.And it was for rooting media,MS NAA 0.1 mg/L,to produce roots from seedings. Taking the stems with single bud as explants on MS 6-BA 1 mg/L NAA 0.05 mg/L,it was found that there are lots of buds after a month.