水稻主要病害拮抗细菌的筛选与鉴定

从稻种、稻叶、稻田土壤、稻田菌核和菜地土壤共 1 0 5份样品中共分离细菌菌株 2 6 72株 ,经拮抗性能测定 ,对水稻纹枯病菌 (Rhizoctoniasolani)、水稻恶苗病菌 (Fusari ummoniliforme)、水稻稻瘟病菌 (Maganporthegrisea)、水稻白叶枯病菌 (Xanthomonasoryzaepv .oryzae)和水稻细菌性条斑病菌 (Xanthomonasoryzaepv .oryzicola)有拮抗作用的菌株分别有 5 0 0、6 7、45、1 1和 9个。根据NA平板上菌落的形态特征 ,将 6 7株稻恶苗病拮抗细菌初步分为 7个类群 ,每一类群中选拮抗性能最强的一个菌株进行鉴定 ,结果有 4支菌株为Bacillussubtilis,另 3支菌株分别为B .pumillus、B .polymyxa和Pseudomonassp

Screening and Identification of Antagonistic Bacteria against Main Rice Diseases

Two thousand six hundred and seventy-two bacteria strains were isolated from 105 samples(rice seeds,rice leaves,paddy soils,sclerotia and vegetable soils),of which,the number of strains with antagonism to Rhizoctonia solani,Fusarium moniliforme,Maganporthe grisea,Xanthomonas oryzae pv.oryzae and Xanthomonas oryzae pv.oryzicola was 500,67,45,11 and 9 respectively.Sixty-seven strains antagonistic to F.moniliforme were classified into 7 groups,one representative strain of each group was identified,the results showed that four strains(31-80,73-95,27-45 and 45-77) were Bacillus subtilis ,and other three strains(3-12,97-49 and 57-13) were B.pumillus,B.polymyxa and Pseudomonas sp. respectively.;