| b型流感嗜血杆菌荚膜多糖纯化方法的优化 |
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| 引用本文： | 尹珊珊,李贵凡,刘学龙,郑海发. b型流感嗜血杆菌荚膜多糖纯化方法的优化[J]. 动物医学进展, 2009, 30(1) |
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| 作者姓名： | 尹珊珊 李贵凡 刘学龙 郑海发 |
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| 作者单位： | 延边大学农学院,吉林,龙井,133400;北京民海生物科技有限公司,北京,102600 |
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| 摘 要： | 对b型流感嗜血杆茵荚膜多糖纯化工艺中冷酚抽提次数、沉淀核酸的乙醇浓度、沉淀糖精的乙醇浓度进行研究.结果表明,最佳纯化工艺为冷酚抽提4次,300 mL/L的乙醇沉淀核酸,650m-L/L的乙醇沉淀糖.所建立的提取纯化工艺提取的荚膜多糖纯度高,各项指标能够达到WHO的规程要求.
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| 关 键 词： | b型流感嗜血杆菌 荚膜多糖 纯化 |
Optimization on Purification of Capsular Polysaccharide from Haemophilus influenzae Type b |
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| YIN Shan-shan,LI Gui-fan,LIU Xue-long,ZHENG Hai-fa. Optimization on Purification of Capsular Polysaccharide from Haemophilus influenzae Type b[J]. Progress In Veterinary Medicine, 2009, 30(1) |
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| Authors: | YIN Shan-shan LI Gui-fan LIU Xue-long ZHENG Hai-fa |
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| Affiliation: | 1.College of Agriculture;Yanbian University;Longjing;Jilin;133400;China;2.Beijing Minhai Biotechnology CO.LTD;Beijing;102600;China |
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| Abstract: | Cool penaol extraction times,ethanol concentration for precipitating nucleic acid and polysaccharide of purification conditions were studied in the article.The results showed that cold phenol extracting 4 times,300 mL/L ehanol precipitating nucleic acid and 650 mL/L ethanol precipitating polysaccharide,respectively,were the optimal conditions,suitable for purification of Haemophilus influenzae type b capsular polysaccharide.The capsular polysaccharide purified accroding to the above conditions had high puri... |
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| Keywords: | Haemophilus influenzae type b capsular polysaccharide purification |
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