| Cloning and molecular evolution research of porcine GAD65 gene |
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| 引用本文： | YU Hao SONG Yuefen LI Li LIU Di. Cloning and molecular evolution research of porcine GAD65 gene[J]. 东北农业大学学报(英文版), 2007, 14(1): 27-30 |
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| 作者姓名： | YU Hao SONG Yuefen LI Li LIU Di |
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| 作者单位： | YU Hao1，SONG Yuefen1，LI Li1，and LIU Di1，2* 1 College of Animal Science and Technology，Northeast Agricultural University，Harbin 150030，China 2 Heilongjiang Academy of Agricultural Sciences，Harbin 150086，China |
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| 基金项目： | Supported in part by Heilongjiang Province Tackle Key Problem Item （GB01B 104） |
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| 摘 要： | Glutamate decarboxylase (GAD) has been found in animal and higher plant tissues as well as in yeasts and microorganisms. In animals the enzyme plays an important role in central nervous system activity because the enzyme substrate glutamic acid is a mediator of excitation process and the product, gamma-aminobutyric acid, is the most important mediator of inhibition process in the central nervous system. GAD65 is one form of the glutamate decarboxylases (GAD), GAD65 has been identified as a major autoantigen in type 1 diabetes, so the GAD65 gene of porcine was cloned by RT-PCR method to construct phylogenetic tree, the homology of 13 glutamate decarboxylases (GAD) of different origin was analyzed by multiple alignment.
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| 关 键 词： | 猪GAD65基因 保守性片段 克隆 分子进化 系统演化 |
| 文章编号： | 1006-8104（2007）-01-0027-04 |
| 收稿时间： | 2006-11-06 |
Cloning and molecular evolution research of porcine GAD65 gene |
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| YU Hao,SONG Yuefen,LI Li,LIU Di. Cloning and molecular evolution research of porcine GAD65 gene[J]. Journal of Northeast Agricultural University, 2007, 14(1): 27-30 |
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| Authors: | YU Hao SONG Yuefen LI Li LIU Di |
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| Abstract: | Glutamate decarboxylase (GAD) has been found in animal and higher plant tissues as well as in yeasts and microorganisms. In animals the enzyme plays an important role in central nervous system activity because the enzyme substrate glutamic acid is a mediator of excitation process and the product, gamma-aminobutyric acid, is the most important mediator of inhibition process in the central nervous system. GAD65 is one form of the glutamate decarboxylases (GAD), GAD65 has been identified as a major autoantigen in type 1 diabetes, so the GAD65 gene of porcine was cloned by RT-PCR method to construct phylogenetic tree, the homology of 13 glutamate decarboxylases (GAD) of different origin was analyzed by multiple alignment. |
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| Keywords: | GAD65 phylogeny conserved residues divergent and convergent evolution |
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